Anti-DNAH6抗体(ab122333)
Key features and details
- Rabbit polyclonal to DNAH6
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DNAH6抗体 -
描述
兔多克隆抗体to DNAH6 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
TKLNTILCQT FVFCYLWSLG GNLTENYYDS FDTFIRTQFD DNPDARLPNS GDLWSIHMDF DTKRLDPWER IIPTFKYNRD VP internal sequence amino acids 1983-2064 of Human DNAH6 (Q9C0G6).
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阳性对照
- Human fallopian tube tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122333于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/850. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/850. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. -
组织特异性
Detected in brain, testis and trachea. -
序列相似性
Belongs to the dynein heavy chain family. -
结构域
Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function. -
细胞定位
Cytoplasm > cytoskeleton > cilium axoneme. - Information by UniProt
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数据库链接
- Entrez Gene: 1768 Human
- Omim: 603336 Human
- SwissProt: Q9C0G6 Human
- Unigene: 249972 Human
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别名
- axonemal antibody
- Axonemal beta dynein heavy chain 6 antibody
- Ciliary dynein heavy chain 6 antibody
see all
图片
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122333 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122333, at a 1/850 dilution, staining DNAH6 in glandular cells of paraffin-embedded Human fallopian tube tissue by immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab122333 被引用在 2 文献中.
- Tu C et al. Bi-allelic mutations of DNAH10 cause primary male infertility with asthenoteratozoospermia in humans and mice. Am J Hum Genet 108:1466-1477 (2021). PubMed: 34237282
- Tu C et al. Identification of DNAH6 mutations in infertile men with multiple morphological abnormalities of the sperm flagella. Sci Rep 9:15864 (2019). PubMed: 31676830