Application
Western blot
Sample
Human Cell lysate - whole cell (A549 tumour cell line)
Loading amount
50 µg
Specification
A549 tumour cell line
Treatment
100IU bleomycin for 2h
Gel Running Conditions
Reduced Denaturing (3-8% tris acetate gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% MILK IN TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 470 kDa Non-specific: none observed kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 27 2009