重组Anti-DKK1抗体[EPR4759] - Low endotoxin,Azide free (ab221779)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4759] to DKK1 - Low endotoxin, Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
概述
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产品名称
Anti-DKK1抗体[EPR4759] - Low endotoxin,Azide free
参阅全部 DKK1 一抗 -
描述
兔单克隆抗体[EPR4759] to DKK1 - Low endotoxin,Azide free -
宿主
Rabbit -
特异性
Although our internal data shows that this antibody is not suitable to detect DKK1 in rats, this antibody has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human placenta and liver tissue WB: Caco-2, PC-3, U-2 OS, MOLT4, HEK-293 cell lysate
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常规说明
ab221779 is the carrier-free version of ab109416.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4759 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221779于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. antigen retrieval is recommended. |
靶标
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功能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
组织特异性
Placenta. -
序列相似性
Belongs to the dickkopf family. -
结构域
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 22943 Human
- Omim: 605189 Human
- SwissProt: O94907 Human
- Unigene: 40499 Human
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别名
- Dickkopf 1 antibody
- Dickkopf 1 homolog antibody
- Dickkopf 1 like antibody
see all
图片
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All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : U-2 OS cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : HEK-293 cell lysate
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 30-45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-DKK1 antibody [EPR4759] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109416).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109416).
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (4)
ab221779 被引用在 4 文献中.
- Li Y et al. Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomed Rep 3:19-24 (2015). WB ; Rat . PubMed: 25469240
- Lyros O et al. Dickkopf-1, the Wnt antagonist, is induced by acidic pH and mediates epithelial cellular senescence in human reflux esophagitis. Am J Physiol Gastrointest Liver Physiol 306:G557-74 (2014). IF ; Human . PubMed: 24481601
- Chen C et al. Elevated levels of Dickkopf-1 are associated with ß-catenin accumulation and poor prognosis in patients with chondrosarcoma. PLoS One 9:e105414 (2014). WB, IHC-P ; Human . PubMed: 25144498
- Dees C et al. The Wnt antagonists DKK1 and SFRP1 are downregulated by promoter hypermethylation in systemic sclerosis. Ann Rheum Dis N/A:N/A (2013). WB . PubMed: 23698475