Synthetic peptide within Human DKK1 aa 250-350. The exact sequence is proprietary.
WB: A549, PC-3, NCI-H460, DU145 and SK-OV-3 whole cell lysates
IHC-P: Human placenta and liver tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Belongs to the dickkopf family.
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution (purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates Lane 3 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates Lane 4 : DU145 (Human prostate carcinoma epithelial cell) whole cell lysates Lane 5 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
The smear bands detected by ab109416 are similar to those reported by the papers (PMID: 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1.
You S et al. Tunicamycin inhibits colon carcinoma growth and aggressiveness via modulation of the ERK-JNK-mediated AKT/mTOR signaling pathway. Mol Med Rep17:4203-4212 (2018).
Read more (PubMed: 29344654) »
Byeon H et al. Long-term prognostic impact of osteopontin and Dickkopf-related protein 1 in patients with hepatocellular carcinoma after hepatectomy. Pathol Res Pract214:814-820 (2018).
Read more (PubMed: 29753515) »