概述

  • 产品名称

    Anti-dimethyl Arginine抗体[21C7]
  • 描述

    小鼠单克隆抗体[21C7] to dimethyl Arginine
  • 宿主

    Mouse
  • 特异性

    This antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.
  • 经测试应用

    适用于: IP, WB, ChIPmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Asymmetric NG -N G -dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)

  • 常规说明

    This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.

性能

应用

Our Abpromise guarantee covers the use of ab413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
WB 1/50 - 1/500.
ChIP Use at an assay dependent concentration. Use 5µl for 4x106 cells.

靶标

  • 别名

    • Di methyl Arginine antibody

文献

This product has been referenced in:

  • Albrecht LV  et al. Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking. Proc Natl Acad Sci U S A 115:E5317-E5325 (2018). Read more (PubMed: 29773710) »
  • Yaseen I  et al. Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3. Nat Commun 6:8922 (2015). Read more (PubMed: 26568365) »
See all 9 Publications for this product

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Application
ChIP
Sample
Human Cell lysate - nuclear (HL-60 cells)
Specification
HL-60 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Taqman
Positive control
input DNA
Negative control
No antibody controls.

Dr. Balint Laszlo Balint

Verified customer

提交于 Oct 04 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

注册