Anti-DGAT1抗体(ab100982)
Key features and details
- Rabbit polyclonal to DGAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-DGAT1抗体
参阅全部 DGAT1 一抗 -
描述
兔多克隆抗体to DGAT1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human DGAT1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab111783) -
阳性对照
- This antibody gave a positive signal in Caco2 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100982于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
靶标
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功能
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. -
通路
Lipid metabolism; glycerolipid metabolism. -
序列相似性
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 282609 Cow
- Entrez Gene: 8694 Human
- Entrez Gene: 13350 Mouse
- Entrez Gene: 397118 Pig
- Entrez Gene: 84497 Rat
- Omim: 604900 Human
- SwissProt: Q8MK44 Cow
- SwissProt: O75907 Human
see all -
别名
- ACAT related gene product 1 antibody
- ACAT-related gene product 1 antibody
- Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
see all
图片
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Western blot - Anti-DGAT1 antibody (ab100982)Anti-DGAT1 antibody (ab100982) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100982 被引用在 1 文献中.
- Guo Z et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619