重组Anti-DFNA5/GSDME抗体[EPR19859-60] - C-terminal (ab221843)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - C-terminal
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DFNA5/GSDME抗体[EPR19859-60] - C-terminal
参阅全部 DFNA5/GSDME 一抗 -
描述
兔单克隆抗体[EPR19859-60] to DFNA5/GSDME - C-terminal -
宿主
Rabbit -
特异性
Expression levels of the target protein vary with sample type and some optimisation may be required.
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经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate. HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate. Human fetal brain lysate. IP: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19859-60 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221843于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 36, 55 kDa (predicted molecular weight: 54 kDa).
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IP |
1/40.
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说明 |
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WB
1/1000. Detects a band of approximately 36, 55 kDa (predicted molecular weight: 54 kDa). |
IP
1/40. |
靶标
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组织特异性
Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta. -
疾病相关
Defects in DFNA5 are the cause of deafness autosomal dominant type 5 (DFNA5) [MIM:600994]. DFNA5 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
序列相似性
Belongs to the gasdermin family. - Information by UniProt
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数据库链接
- Entrez Gene: 1687 Human
- Omim: 608798 Human
- SwissProt: O60443 Human
- Unigene: 520708 Human
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别名
- 2310037D07Rik antibody
- 4932441K13Rik antibody
- Deafness, autosomal dominant 5 antibody
see all
图片
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All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/5000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 2 : DFNA5/GSDME knockout SH-SY5Y, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Exposure time: 30 secondsBlocking/Dilution: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.
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All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with a control vector containing DDDDK tag, whole cell lysate
Lane 2 : HEK-293 transfected with a DDDDK tagged, DFNA5/GSDME (N-terminus) expression vector, whole cell lysate
Lane 3 : HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 5 secondsBlocking/Dilution: 5% NFDM/TBST .
This antibody is specific to the C-terminus of DFNA5/GSDME.
The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.
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Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution + human fetal brain at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutesBlocking/Dilution: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28459430).
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DFNA5/GSDME was immunoprecipitated from 0.35 mg of SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab221843 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab221843 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: SH-SY5Y whole cell lysate 10ug (Input).
Lane 2: ab221843 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221843 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Observed MW 55 kDa.
Exposure time: 1 second.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab221843 被引用在 1 文献中.
- Wang Y et al. Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a Gasdermin. Nature N/A:N/A (2017). PubMed: 28459430