Anti-Desmoglein 2/DSG2抗体- C-terminal (ab226258)
Key features and details
- Rabbit polyclonal to Desmoglein 2/DSG2 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Desmoglein 2/DSG2抗体- C-terminal
参阅全部 Desmoglein 2/DSG2 一抗 -
描述
兔多克隆抗体to Desmoglein 2/DSG2 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rat, Chimpanzee, Cynomolgus monkey, Rhesus monkey -
免疫原
Synthetic peptide within Human Desmoglein 2/DSG2 aa 1068-1118 (C terminal). The exact sequence is proprietary. (NP_001934.2).
Database link: Q14126 -
阳性对照
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab226258 was affinity purified using an epitope specific to Desmoglein 2/DSG2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 122 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
组织特异性
All of the tissues tested and carcinomas. -
疾病相关
Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Contains 4 cadherin domains. -
结构域
Calcium may be bound by the cadherin-like repeats. -
细胞定位
Cell membrane. Cell junction > desmosome. - Information by UniProt
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数据库链接
- Entrez Gene: 455356 Chimpanzee
- Entrez Gene: 1829 Human
- Entrez Gene: 307562 Rat
- Omim: 125671 Human
- SwissProt: Q14126 Human
- Unigene: 412597 Human
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别名
- ARVC 10 antibody
- ARVC10 antibody
- ARVD 10 antibody
see all
图片
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All lanes : Anti-Desmoglein 2/DSG2 antibody - C-terminal (ab226258) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3 seconds -
Desmoglein 2/DSG2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226258 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226258 at 1 µg/ml.
Lane 1: ab226258 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226258 尚未被引用在任何文献中。