Anti-Desmin抗体(ab8592)
Key features and details
- Rabbit polyclonal to Desmin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Desmin抗体
参阅全部 Desmin 一抗 -
描述
兔多克隆抗体to Desmin -
宿主
Rabbit -
特异性
In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas. -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length protein corresponding to Human Desmin.
Database link: P17661 -
常规说明
Prolonged fixation in buffered formalin can destroy the epitope.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.01% PBS, 0.2% BSA -
Concentration information loading...
-
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB | (5) |
Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
|
说明 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa. |
靶标
-
功能
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
疾病相关
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
序列相似性
Belongs to the intermediate filament family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P17661 Human
- SwissProt: P31001 Mouse
- SwissProt: P48675 Rat
- Unigene: 594952 Human
see all -
别名
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
图片
-
All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution
Lane 1 : RMS13 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human heart tissue lysate
Lane 5 : C2C12 cell lysate
Lane 6 : Mouse skeletal muscle tissue lysate
Lane 7 : Mouse heart tissue lysate
Lane 8 : L6 cell lysate
Lane 9 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
数据表及文件
-
SDS download
-
Datasheet download
文献 (95)
ab8592 被引用在 95 文献中.
- Uemura L et al. Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction. J Funct Biomater 13:N/A (2022). PubMed: 35645267
- Melnikov IY et al. Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle. Pflugers Arch 474:1171-1183 (2022). PubMed: 35931829
- Cordero-Espinoza L et al. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation. Cell Stem Cell 28:1907-1921.e8 (2021). PubMed: 34343491
- Filomena MC et al. Myopalladin knockout mice develop cardiac dilation and show a maladaptive response to mechanical pressure overload. Elife 10:N/A (2021). PubMed: 34558411
- Blume GG et al. Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure. Life (Basel) 11:N/A (2021). PubMed: 34575107