Anti-Desmin抗体(ab227224)
Key features and details
- Rabbit polyclonal to Desmin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Desmin抗体
参阅全部 Desmin 一抗 -
描述
兔多克隆抗体to Desmin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Cow, Dog, Pig, Rhesus monkey -
免疫原
Recombinant fragment within Human Desmin (internal sequence). The exact sequence is proprietary.
Database link: P17661 -
阳性对照
- WB: Rat2, RD, A549 and HCT116 whole cell lysate; mouse muscle tissue lysate. IHC-P: Mouse smooth muscle tissue and skeletal muscle tissue; H1299 xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227224于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 54 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/1000. Predicted molecular weight: 54 kDa. |
靶标
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功能
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
疾病相关
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
序列相似性
Belongs to the intermediate filament family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 280765 Cow
- Entrez Gene: 497091 Dog
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 396725 Pig
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: O62654 Cow
see all -
别名
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
图片
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Paraffin-embedded mouse smooth muscle tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.
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Anti-Desmin antibody (ab227224) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Anti-Desmin antibody (ab227224) at 1/500 dilution + RD (human rhabdomyosarcoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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All lanes : Anti-Desmin antibody (ab227224) at 1/3000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Anti-Desmin antibody (ab227224) at 1/1000 dilution + Mouse muscle tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Paraffin-embedded mouse skeletal muscle tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded NCI-H1299 (human lung carcinoma cell line) xenograft tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227224 尚未被引用在任何文献中。