概述

  • 产品名称
  • 描述
    兔多克隆抗体to DEPDC5
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment corresponding to Human DEPDC5 aa 462-548.
    Sequence:

    YDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTL PTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSS


    Database link: O75140

  • 阳性对照
    • Human stomach tissue.

性能

应用

Our Abpromise guarantee covers the use of ab185565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

靶标

图片

  • Ab185565 Immunofluorescent staining of human cell line HeLa shows positivity in the Golgi apparatus. Antibody staining is shown in green.

文献

ab185565 has not yet been referenced specifically in any publications.

客户评价及客户问答

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1-3 of 3 Abreviews

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (gel 3-8%)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Alexandre Bacq

Verified customer

提交于 Aug 03 2018

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 knock down cells)
Gel Running Conditions
Reduced Denaturing (6% SDA PAGE)
Loading amount
60 µg
Specification
HepG2 knock down cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Mar 07 2018

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7, Breast cancer cells)
Gel Running Conditions
Reduced Denaturing (8 %)
Loading amount
50 µg
Specification
MCF7, Breast cancer cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Jul 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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