Anti-DEPDC5抗体(ab185565)

概述

  • 产品名称
    Anti-DEPDC5抗体
  • 描述
    兔多克隆抗体to DEPDC5
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment corresponding to Human DEPDC5 aa 462-548.
    Sequence:

    YDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTL PTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSS


    Database link: O75140

  • 阳性对照
    • Human stomach tissue.

性能

应用

Our Abpromise guarantee covers the use of ab185565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • Ab185565 Immunofluorescent staining of human cell line HeLa shows positivity in the Golgi apparatus. Antibody staining is shown in green.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DEPDC5 with ab185565 at 1/200 dilution.

文献

ab185565 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 knock down cells)
Gel Running Conditions
Reduced Denaturing (6% SDA PAGE)
Loading amount
60 µg
Specification
HepG2 knock down cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 07 2018

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7, Breast cancer cells)
Gel Running Conditions
Reduced Denaturing (8 %)
Loading amount
50 µg
Specification
MCF7, Breast cancer cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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