重组Anti-DENN抗体[EPR4919] (ab134117)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4919] to DENN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-DENN抗体[EPR4919]
参阅全部 DENN 一抗 -
描述
兔单克隆抗体[EPR4919] to DENN -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cell lysate, 293T cell lysate, K562 cell lysate, NIH 3T3 cell lysate, Human breast tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 7.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4919 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134117于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 183 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 183 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect. -
组织特异性
Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. -
序列相似性
Belongs to the MADD family.
Contains 1 dDENN domain.
Contains 1 death domain.
Contains 1 DENN domain.
Contains 1 uDENN domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8567 Human
- Entrez Gene: 228355 Mouse
- Entrez Gene: 94193 Rat
- Omim: 603584 Human
- SwissProt: Q8WXG6 Human
- SwissProt: Q80U28 Mouse
- SwissProt: O08873 Rat
- Unigene: 82548 Human
see all -
别名
- Differentially expressed in normal and neoplastic cells antibody
- FLJ35600 antibody
- IG20 antibody
see all
图片
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All lanes : Anti-DENN antibody [EPR4919] (ab134117) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : K562 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 183 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Human breast tissue labelling DENN with ab134117 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab134117 被引用在 2 文献中.
- Schneeberger PE et al. Biallelic MADD variants cause a phenotypic spectrum ranging from developmental delay to a multisystem disorder. Brain 143:2437-2453 (2020). PubMed: 32761064
- Xu F et al. KIF1Bß mutations detected in hereditary neuropathy impair IGF1R transport and axon growth. J Cell Biol 217:3480-3496 (2018). PubMed: 30126838