产品名称Anti-delta 1 Catenin/CAS (phospho T310)抗体[EPR2382]
参阅全部 delta 1 Catenin/CAS 一抗
描述兔单克隆抗体[EPR2382] to delta 1 Catenin/CAS (phospho T310)
特异性The antibody only detects delta 1 Catenin when phosphorylated on Threonine 310.
经测试应用适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide within Human delta 1 Catenin/CAS (phospho T310). The exact sequence is proprietary.
- 293T cell lysates treated with calyculin A Human breast carcinoma tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab81318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 108 kDa.|
|IHC-P||1/100 - 1/250.|
功能Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
组织特异性Expressed in vascular endothelium.
序列相似性Belongs to the beta-catenin family.
Contains 10 ARM repeats.
结构域A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
翻译后修饰Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
细胞定位Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
- Information by UniProt
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
All lanes : Anti-delta 1 Catenin/CAS (phospho T310) antibody [EPR2382] (ab81318) at 1/5000 dilution
Lane 1 : 293T cell lysates untreated
Lane 2 : 293T cell lysates treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab81318 at 1/100 dilution.
ab81318 has not yet been referenced specifically in any publications.