重组Anti-delta 1 Catenin/CAS抗体[EPR357(2)] (ab92514)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR357(2)] to delta 1 Catenin/CAS
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-delta 1 Catenin/CAS抗体[EPR357(2)]
参阅全部 delta 1 Catenin/CAS 一抗 -
描述
兔单克隆抗体[EPR357(2)] to delta 1 Catenin/CAS -
宿主
Rabbit -
特异性
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
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经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human delta 1 Catenin/CAS aa 950 to the C-terminus. The exact sequence is proprietary.
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阳性对照
- WB: HeLa, HEK-293T, RAW264.7, C2C12 and A431 cell lysates. IHC-P: Human breast carcinoma, ovarian cancer and colonic adenocarcinoma tissues. ICC/IF: A431 cells. Flow Cyt (intra): A431 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 7.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR357(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92514于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (6) |
1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
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Flow Cyt (Intra) |
1/20.
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ICC/IF | (3) |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 108 kDa. |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
Flow Cyt (Intra)
1/20. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. -
组织特异性
Expressed in vascular endothelium. -
序列相似性
Belongs to the beta-catenin family.
Contains 10 ARM repeats. -
结构域
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted. -
翻译后修饰
Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1500 Human
- Entrez Gene: 12388 Mouse
- Omim: 601045 Human
- SwissProt: O60716 Human
- SwissProt: P30999 Mouse
- Unigene: 166011 Human
- Unigene: 35738 Mouse
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别名
- Cadherin associated Src substrate antibody
- Cadherin-associated Src substrate antibody
- CAS antibody
see all
图片
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All lanes : Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 3 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?The multiple bands are isoforms caused by alternatively splicing as was described in PMID 9653641 and are consistent with what has been descirbed in PMID 15077190.
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Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue sections labeling delta 1 Catenin/CAS with purified ab92514 at 1:50 dilution (1.84 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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A431 (Human epidermoid carcinoma cell line) cells stained for delta 1 Catenin/CAS using unpurified ab92514 at a dilution of 1/100 in ICC/IF.
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Paraffin embedded human breast carcinoma tissue (panel 1) and human colonic adenocarcinoma tissue (panel 2) labeled with unpurified ab92514 at 1/100 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin/CAS with unpurified ab92514 at 1/20 dilution (10µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab92514 被引用在 17 文献中.
- Liu Y et al. Downregulated circular RNA hsa_circ_0005797 inhibits endometrial cancer by modulating microRNA-298/Catenin delta 1 signaling. Bioengineered 13:4634-4645 (2022). PubMed: 34852711
- Wu Y et al. Ruscogenin alleviates LPS-triggered pulmonary endothelial barrier dysfunction through targeting NMMHC IIA to modulate TLR4 signaling. Acta Pharm Sin B 12:1198-1212 (2022). PubMed: 35530141
- Ben Kacem M et al. Variation of 4 MV X-ray dose rate strongly impacts biological response both in vitro and in vivo. Sci Rep 10:7021 (2020). PubMed: 32341396
- Raghu D et al. GALNT3 Maintains the Epithelial State in Trophoblast Stem Cells. Cell Rep 26:3684-3697.e7 (2019). PubMed: 30917321
- Ding X et al. MicroRNA-143-3p suppresses tumorigenesis by targeting catenin-d1 in colorectal cancer. Onco Targets Ther 12:3255-3265 (2019). PubMed: 31118676