Anti-DDX56/DDX21抗体(ab97648)
Key features and details
- Rabbit polyclonal to DDX56/DDX21
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DDX56/DDX21抗体
参阅全部 DDX56/DDX21 一抗 -
描述
兔多克隆抗体to DDX56/DDX21 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human DDX56/DDX21 aa 251-403. (AAH01235)
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阳性对照
- A549, HeLa, HepG2 and HCT116 cell lines; MCF7 xenograft ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97648于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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ICC/IF |
1/100 - 1/500.
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说明 |
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IHC-P
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
ICC/IF
1/100 - 1/500. |
靶标
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功能
May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity. -
组织特异性
Detected in heart, brain, liver, pancreas, placenta and lung. -
序列相似性
Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 508728 Cow
- Entrez Gene: 54606 Human
- Entrez Gene: 52513 Mouse
- Entrez Gene: 289780 Rat
- Omim: 608023 Human
- SwissProt: Q3SZ40 Cow
- SwissProt: Q9NY93 Human
- SwissProt: Q9D0R4 Mouse
see all -
别名
- 61 kd nucleolar helicase antibody
- ATP-dependent 61 kDa nucleolar RNA helicase antibody
- DDX21 antibody
see all
图片
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Paraformaldehyde-fixed HeLa cells stained for DDX56/DDX21 using ab97648 at 1/500 dilution in ICC/IF (green). Alpha tubulin filaments are detected with an ant-tubulin antibody (red).
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Anti-DDX56/DDX21 antibody (ab97648) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE -
ab97648, at a 1/200 dilution, staining DDX56/DDX21 in paraformaldehyde fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
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ab97648, at a 1/500 dilution, staining DDX56/DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97648 被引用在 1 文献中.
- Li D et al. DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import. J Cell Sci 133:N/A (2019). PubMed: 31340999