Anti-DDX4 / MVH抗体(ab13840)
Key features and details
- Rabbit polyclonal to DDX4 / MVH
- Suitable for: WB, ICC/IF, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-DDX4 / MVH抗体
参阅全部 DDX4 / MVH 一抗 -
描述
兔多克隆抗体to DDX4 / MVH -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Sheep, Cow, Pig, Platypus -
免疫原
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阳性对照
- ICC: mES cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13840于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841).
|
ICC/IF | (2) |
Use a concentration of 1 µg/ml.
|
IHC-P | (11) |
Use at an assay dependent concentration.
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IHC-Fr | (7) |
Use at an assay dependent concentration.
|
说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841). |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. -
组织特异性
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. -
序列相似性
Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. - Information by UniProt
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数据库链接
- Entrez Gene: 493725 Cow
- Entrez Gene: 54514 Human
- Entrez Gene: 13206 Mouse
- Entrez Gene: 310090 Rat
- Omim: 605281 Human
- SwissProt: Q5W5U4 Cow
- SwissProt: Q9NQI0 Human
- SwissProt: Q61496 Mouse
see all -
别名
- DDX 4 antibody
- Ddx4 antibody
- DDX4_HUMAN antibody
see all
图片
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ab13840 staining DDX4/MVH in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab13840 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Mouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1
g/ml) overnight at 4 degrees. ab13840 staining is shown in red and Oct4 staining is shown in green.µ The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.
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All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 28 kDa, 47 kDa. We are unsure as to the identity of these extra bands. -
All lanes : ab13840 at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 52 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse Ovary Lysate at 10 µg
Lane 2 : Mouse Embryonic Germ Cell Lysate at 20 µg
Lane 3 : Mouse Ovary Lysate at 10 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell Lysate at 20 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Predicted band size: 76 kDa
Observed band size: 76 kDa -
IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
数据表及文件
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SDS download
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Datasheet download
文献 (487)
ab13840 被引用在 487 文献中.
- Jung SE et al. Autophagy modulation alleviates cryoinjury in murine spermatogonial stem cell cryopreservation. Andrology 10:340-353 (2022). PubMed: 34499811
- Zuo Q et al. Dual regulatory actions of LncBMP4 on BMP4 promote chicken primordial germ cell formation. EMBO Rep 23:e52491 (2022). PubMed: 34747116
- Kato T et al. Low Serum Inhibin B/Follicle-Stimulating Hormones and Anti-Müllerian Hormone/Follicle-Stimulating Hormones Ratios as Markers of Decreased Germ Cells in Infants with Bilateral Cryptorchidism. J Urol 207:701-709 (2022). PubMed: 34823367
- Thomson E et al. Generation and mutational analysis of a transgenic mouse model of human SRY. Hum Mutat 43:362-379 (2022). PubMed: 34918413
- Liu R et al. SETDB1 Regulates Porcine Spermatogonial Adhesion and Proliferation through Modulating MMP3/10 Transcription. Cells 11:N/A (2022). PubMed: 35159180