Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(HRP) (ab1238)

概述

  • 产品名称

    Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(HRP)
    参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗
  • 描述

    山羊多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence) (HRP)
  • 宿主

    Goat
  • 偶联物

    HRP
  • 经测试应用

    适用于: ELISA, WB, ICCmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Full length native protein (purified) corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 常规说明

    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/100000.
WB 1/1000 - 1/30000.
ICC 1/200 - 1/500.

靶标

  • 相关性

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 别名

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

图片

  • Anti-FLAG® immunostaining (using ab1238) of ntl:FLAG® BAC transgenic zebrafish embryos shows expression in the margin at 6 hours post fertilisation.

文献

This product has been referenced in:

  • Feng S  et al. Tripartite motif-containing 14 (TRIM14) promotes epithelial-mesenchymal transition via ZEB2 in glioblastoma cells. J Exp Clin Cancer Res 38:57 (2019). Read more (PubMed: 30728039) »
  • Emmer BT  et al. The cargo receptor SURF4 promotes the efficient cellular secretion of PCSK9. Elife 7:N/A (2018). Read more (PubMed: 30251625) »
See all 11 Publications for this product

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