Key features and details
- Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence) (HRP)
- Suitable for: ELISA, WB, ICC
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
产品名称Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(HRP)
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗
描述山羊多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence) (HRP)
经测试应用适用于: ELISA, WB, ICCmore details
种属反应性与反应: Species independent
Full length native protein (purified) corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
存放说明Shipped at 4°C. Store at +4°C.
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
Concentration information loading...
纯度Immunogen affinity purified
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab1238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/10000 - 1/100000.|
|WB||1/1000 - 1/30000.|
|ICC||1/200 - 1/500.|
相关性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
ab1238 被引用在 14 文献中.
- Feng S et al. Tripartite motif-containing 14 (TRIM14) promotes epithelial-mesenchymal transition via ZEB2 in glioblastoma cells. J Exp Clin Cancer Res 38:57 (2019). PubMed: 30728039
- Graindorge A et al. In-cell identification and measurement of RNA-protein interactions. Nat Commun 10:5317 (2019). PubMed: 31757954
- Volpe M et al. Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11. FASEB J 33:9752-9761 (2019). PubMed: 31162950
- Emmer BT et al. The cargo receptor SURF4 promotes the efficient cellular secretion of PCSK9. Elife 7:N/A (2018). PubMed: 30251625
- Mao SQ et al. DNA G-quadruplex structures mold the DNA methylome. Nat Struct Mol Biol 25:951-957 (2018). PubMed: 30275516