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I would like to detect FLAG (DDDDK) by immunohistology and wanted to know if you have an antibody that you would recommend for paraffin fixed sections. We work on bone so frozen sections are a bit of a nightmare for us.
Asked on Jan 23 2006
Thank you for your enquiry. None of our FLAG tag antibodies have currently been tested in IHC on paraffin sections. Some have been tested on frozen sections (ab1257,ab21536) and as this work in this application this indicates that those antibodies have the ability to recognise the native form of the protein so have good chances of working in other immunohistochemical methods. They therefore have a chance of working in IHC-P, providing the fixation of the tissue does not modify the epitope dramatically and prevent recognition by the antibodies. I hope this information will help you, please do not hesitate to contact me again if you need more advice,
回复于 Jan 24 2006