Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(ab1170)

概述

  • 产品名称

    Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体
    参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗
  • 描述

    鸡多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence)
  • 宿主

    Chicken
  • 经测试应用

    适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • 常规说明

    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antibodies were affinity purified using the peptide coupled to a different carrier.
  • 克隆

    多克隆
  • 同种型

    IgY
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab1170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/20000.
WB 1/2000 - 1/10000.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 别名

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

图片

  • ab1170 staining DDDDK in Mouse primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 1x PBS + 5% Goat serum + 0.1% Triton-X 100) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-chicken IgY polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170) at 1/5000 dilution
    Secondary
    Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 66 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds

    See Abreview

文献

This product has been referenced in:

  • Lim J  et al. Nuclear Proximity of Mtr4 to RNA Exosome Restricts DNA Mutational Asymmetry. Cell 169:523-537.e15 (2017). Read more (PubMed: 28431250) »
  • Hutson MR  et al. Temperature-activated ion channels in neural crest cells confer maternal fever-associated birth defects. Sci Signal 10:N/A (2017). Read more (PubMed: 29018170) »
See all 2 Publications for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary hippocampal neurons)
Permeabilization
No
Specification
Primary hippocampal neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Oct 18 2013

Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21)
Gel Running Conditions
Reduced Denaturing (4~20%)
Loading amount
0.1 µg
Specification
BL21
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Sep 30 2010

Application
Western blot
Sample
Escherichia coli Purified protein (Mach1cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
0.5 µg
Specification
Mach1cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Sep 09 2009

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