Application
Western blot
Sample
Rabbit Cell lysate - whole cell (HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: swine anti-rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Untransfected cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 48 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Emil Kofod-Olsen
Verified customer
提交于 Dec 21 2007