产品名称Anti-DDDDK tag抗体- (Equivalent to FLAG抗体from Sigma)
参阅全部 DDDDK tag 一抗
描述山羊多克隆抗体to DDDDK tag - (Equivalent to FLAG抗体from Sigma)
经测试应用适用于: ELISA, WB, ICC/IFmore details
常规说明Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.1% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab1257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/100 - 1/500.|
|WB||1/1000 - 1/30000.|
|ICC/IF||1/200 - 1/2000.|
相关性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
Human microvascular endothelial cells expressing DDDDK-tagged beta-catenin following transient transfection.
Ab1257 used at 1 µg/ml. Detected with FITC labeled rabbit anti-goat IgG (H&L) secondary antibody.
All lanes : Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1257) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate. at 0.2 µg
Lane 2 : E. coli whole cell lysate. at 0.1 µg
Lane 3 : E. coli whole cell lysate. at 0.05 µg
Western Blot analysis of E. coli Whole Cell Lysates, labelled with DDDDK-tagged Protein by ab1257 at 1/25,000.
This product has been referenced in:
- Martin LJ et al. Electric fields control the orientation of peptides irreversibly immobilized on radical-functionalized surfaces. Nat Commun 9:357 (2018). Read more (PubMed: 29367659) »
- Gupta S et al. Herpesvirus deconjugases inhibit the IFN response by promoting TRIM25 autoubiquitination and functional inactivation of the RIG-I signalosome. PLoS Pathog 14:e1006852 (2018). Read more (PubMed: 29357390) »