Anti-DDDDK tag抗体(Agarose) (ab1240)


  • 产品名称
    Anti-DDDDK tag抗体(Agarose)
    参阅全部 DDDDK tag 一抗
  • 描述
    山羊多克隆抗体to DDDDK tag (Agarose)
  • 宿主
  • 偶联物
  • 经测试应用
    适用于: IPmore details
  • 免疫原

    Full length native protein (purified) corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).


  • 常规说明
    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.



Our Abpromise guarantee covers the use of ab1240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.


  • 相关性
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • 别名
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all


This product has been referenced in:
  • Hughes DJ  et al. NEDDylation is essential for Kaposi's sarcoma-associated herpesvirus latency and lytic reactivation and represents a novel anti-KSHV target. PLoS Pathog 11:e1004771 (2015). IP ; Human . Read more (PubMed: 25794275) »
  • Klement K  et al. Opposing ISWI- and CHD-class chromatin remodeling activities orchestrate heterochromatic DNA repair. J Cell Biol 207:717-33 (2014). Read more (PubMed: 25533843) »

See all 3 Publications for this product


Thank you for contacting us.

If you are doing co-IP, it is not recommended to use RIPA buffer as it denaturing and may be too harsh on your protein complexes. I would suggest using a non-denaturing lysis buffer. For example:

20 mM T...

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Thank you for your reply.

I would recommend for the first time use of using a couple of different antibody concentrations and then seeing which one gives the best signal. You could have one condition where you use 2ug of antibody and another ...

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Thank you for your reply.

This product are ready to use and the total volume of each vial you receive should be 400ul (200ul of gel and 200ul of buffer). Therefore to get a 50% slurry, you would have to use 100ul of product, wash in PBS and t...

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Thank you for contacting Abcam.

You would use the product, ab1240 as you would use regular Protein A/G Sepharose beads, same type of buffers and centrifugation steps. I have attached the Abcam protocols booklet, which contains a general IP pr...

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Vielen Dank für Ihren Anruf.

Da es für Sie so dringend ist, habe ich im Labor angerufen, und man konnte mir tatsächlich prompt Auskunft geben:

Die Konzentration beträgt 10 ug Antikoerper/200 ug "Slurry"...

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Thank you for your inquiry.

Pre-clearing the lysates is always up to the end-user to determine because background levels will vary. Since both of these antibodies are conjugated to agarose, if you want to pre-clear the lysates you could...

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Thank you for contacting us. I am sorry to hear you have been experiencing problems with this anti-DDDDK tag antibody. Indeed, 100 µg of the affinity purified antibody is attached to the agarose gel and the total volume of each vial you received ...

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Thank you for your inquiry. Concentration: 100 ug antibody/200 ul gel Volume: 400 ul of 50% slurry containing 200 ul gel Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG,...

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