Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus lysate)
Loading amount
40 µg
Specification
Hippocampus lysate
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Goat IR700-linked (LiCor, UK)
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Dilution
1/15000
Detection
Detection method
Odyssey Detection System, LiCor UK
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 31 kDa Non-specific: 50, 60, 75 kDa
Additional data
Additional Notes
Try dilution 1/1000-1/3000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Richard D'Mello
Verified customer
提交于 Apr 14 2009