Anti-DDAH1抗体(ab2231)
Key features and details
- Goat polyclonal to DDAH1
- Suitable for: ICC, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-DDAH1抗体
参阅全部 DDAH1 一抗 -
描述
山羊多克隆抗体to DDAH1 -
宿主
Goat -
经测试应用
适用于: ICC, Flow Cyt, WBmore details -
种属反应性
与反应: Human
预测可用于: Cow
-
免疫原
-
阳性对照
- WB: HepG2 and LnCaP cell lysates. Human cerebellum, kidney and testes. ICC: HeLa and U20S cells Flow cyt: HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 10 µg/ml.
|
|
| Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
| WB | (3) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
ICC
Use a concentration of 10 µg/ml. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa). 1 hour primary incubation is recommended for this product. |
靶标
-
功能
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
组织特异性
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. -
序列相似性
Belongs to the DDAH family. - Information by UniProt
-
数据库链接
- Entrez Gene: 23576 Human
- Omim: 604743 Human
- SwissProt: P56965 Cow
- SwissProt: O94760 Human
- Unigene: 379858 Human
- Unigene: 713411 Human
-
别名
- DDAH antibody
- DDAH I antibody
- DDAH-1 antibody
see all
图片
-
Flow Cytometry - Anti-DDAH1 antibody (ab2231)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 1ug/ml. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Immunocytochemistry - Anti-DDAH1 antibody (ab2231)Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody. -
Immunocytochemistry - Anti-DDAH1 antibody (ab2231)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton using ab2231 at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody. -
Western blot - Anti-DDAH1 antibody (ab2231)All lanes : Anti-DDAH1 antibody (ab2231) at 0.3 µg/ml
Lane 1 : Human cerebellum
Lane 2 : Human kidney
Lane 3 : Human testes
Predicted band size: 31 kDa -
Western blot - Anti-DDAH1 antibody (ab2231)All lanes : Anti-DDAH1 antibody (ab2231) at 0.3 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : LnCaP cell lysate
Predicted band size: 31 kDa
实验方案
数据表及文件
-
Datasheet download
文献 (15)
ab2231 被引用在 15 文献中.
- Ding X et al. Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer. Cancer Manag Res 13:1-11 (2021). PubMed: 33442289
- Zhu ZD et al. DDAH2 alleviates myocardial fibrosis in diabetic cardiomyopathy through activation of the DDAH/ADMA/NOS/NO pathway in rats. Int J Mol Med 43:749-760 (2019). PubMed: 30569164
- Joris V et al. MicroRNA-199a-3p and MicroRNA-199a-5p Take Part to a Redundant Network of Regulation of the NOS (NO Synthase)/NO Pathway in the Endothelium. Arterioscler Thromb Vasc Biol 38:2345-2357 (2018). PubMed: 29976767
- Balasubramanian V et al. Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats. Sci Rep 7:17900 (2017). WB ; Rat . PubMed: 29263339
- Hulin JA et al. MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Sci Rep 7:13996 (2017). PubMed: 29070803
