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Neuroscience Neurotransmission Nitric Oxide NOS

Anti-DDAH1抗体(ab2231)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (14)

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Western blot - Anti-DDAH1 antibody (ab2231)

    Key features and details

    • Goat polyclonal to DDAH1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant Human DDAH1 protein (ab99863)

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    概述

    • 产品名称

      Anti-DDAH1抗体
      参阅全部 DDAH1 一抗
    • 描述

      山羊多克隆抗体to DDAH1
    • 宿主

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • 免疫原

      Synthetic peptide corresponding to Human DDAH1 aa 273-285 (C terminal).
      Sequence:

      TCCSVLINKKVDS


      Database link: O94760
      (Peptide available as ab99047)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurotransmission
      • Nitric Oxide
      • NOS
      • Cardiovascular
      • Vasculature
      • Endothelium

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human DDAH1 protein (ab99863)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2231 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Human
    All applications
    Cow
    应用 Ab评论 说明
    WB (3)
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

    1 hour primary incubation is recommended for this product.

    说明
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

    1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
    • 组织特异性

      Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
    • 序列相似性

      Belongs to the DDAH family.
    • Target information above from: UniProt accession O94760 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 23576 Human
      • Omim: 604743 Human
      • SwissProt: P56965 Cow
      • SwissProt: O94760 Human
      • Unigene: 379858 Human
      • Unigene: 713411 Human
      • 别名

        • DDAH antibody
        • DDAH I antibody
        • DDAH-1 antibody
        • DDAH1 antibody
        • DDAH1_HUMAN antibody
        • DDAHI antibody
        • Dimethylargininase 1 antibody
        • Dimethylargininase-1 antibody
        • Dimethylarginine dimethylaminohydrolase 1 antibody
        • N(G) antibody
        • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
        • NG NG dimethylarginine dimethylaminohydrolase antibody
        see all

      图片

      • Western blot - Anti-DDAH1 antibody (ab2231)
        Western blot - Anti-DDAH1 antibody (ab2231)
        Anti-DDAH1 antibody (ab2231) at 0.100000001490116 µg/ml + Human kidney lysate at 35 µg

        Predicted band size: 31 kDa

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (14)

      发表研究结果有使用 ab2231?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2231 被引用在 14 文献中.

      • Zhu ZD  et al. DDAH2 alleviates myocardial fibrosis in diabetic cardiomyopathy through activation of the DDAH/ADMA/NOS/NO pathway in rats. Int J Mol Med 43:749-760 (2019). PubMed: 30569164
      • Joris V  et al. MicroRNA-199a-3p and MicroRNA-199a-5p Take Part to a Redundant Network of Regulation of the NOS (NO Synthase)/NO Pathway in the Endothelium. Arterioscler Thromb Vasc Biol 38:2345-2357 (2018). PubMed: 29976767
      • Balasubramanian V  et al. Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats. Sci Rep 7:17900 (2017). WB ; Rat . PubMed: 29263339
      • Hulin JA  et al. MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Sci Rep 7:13996 (2017). PubMed: 29070803
      • Mookerjee RP  et al. Hepatic dimethylarginine-dimethylaminohydrolase1 is reduced in cirrhosis and is a target for therapy in portal hypertension. J Hepatol 62:325-31 (2015). WB, IHC ; Human . PubMed: 25152204
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-DDAH1 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Brain (Hippocampus))
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      Brain (Hippocampus)
      Blocking step
      Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
      Read More

      圭一 角山

      Verified customer

      提交于 Nov 25 2015

      Western blot abreview for Anti-DDAH1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (hippocampus)
      Loading amount
      5 µg
      Specification
      hippocampus
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Sep 09 2010

      Western blot abreview for Anti-DDAH1 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (Hippocampus lysate)
      Loading amount
      40 µg
      Specification
      Hippocampus lysate
      Gel Running Conditions
      Reduced Denaturing (12% gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Mr. Richard D'Mello

      Verified customer

      提交于 Apr 14 2009

      Question

      Hi! So, we had a customer call today who is using ab 2231 in both human and rat aortic homogenate. He has loaded from 100 ugs to 1 mg of protein per lane diluted the antibody 5 ugs/ml and incubated overnight. He sees a band at 32kDa but nothing at 40 kDa. It says on the datasheet that the predicted MW is 33 kDa but runs at 40 kDa do you have the peptide available for them to run a blocking study? The other issue is that they see this band in their negative control lane (with BSA). Otherwise, this looks to be an issue with the antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 20 2003

      Answer

      According to the datasheet, this antibody is thought to run at 40kDa due glycosylation. It could be that the protein is running at 32kDa in their experiments as the protein in their system is not glycosylated. However, I am concerned that their negative control is also showing a band at the same size. We do not have the peptide here for them to try but I will ask the originator of the antibody if they have the blocking peptide available. It would help if the researcher could contact us directly and provide a blot image. Are they just loading BSA in their negative control lane? Please ask them to contact us by e-mailing technical@abcam.com and attaching a blot image to the e-mail.

      Read More

      Abcam Scientific Support

      回复于 Jun 20 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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