Anti-DCTN1/p150-glued抗体(ab96004)
Key features and details
- Rabbit polyclonal to DCTN1/p150-glued
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-DCTN1/p150-glued抗体
参阅全部 DCTN1/p150-glued 一抗 -
描述
兔多克隆抗体to DCTN1/p150-glued -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1216-1248.
Database link: NP_004073 -
阳性对照
- Jurkat whole cell lysate (ab7899) for WB. HeLa cells for IF. OV CA for IHC-P.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96004于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 142 kDa.
|
|
IHC-P |
1/100 - 1/500.
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 142 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
靶标
-
功能
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. -
组织特异性
Brain. -
疾病相关
Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally. -
序列相似性
Belongs to the dynactin 150 kDa subunit family.
Contains 1 CAP-Gly domain. -
翻译后修饰
Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
-
数据库链接
- Entrez Gene: 1639 Human
- Entrez Gene: 13191 Mouse
- Entrez Gene: 29167 Rat
- Omim: 601143 Human
- SwissProt: Q14203 Human
- SwissProt: O08788 Mouse
- SwissProt: P28023 Rat
- Unigene: 516111 Human
see all -
别名
- 150 kDa dynein associated polypeptide antibody
- 150 kDa dynein-associated polypeptide antibody
- DAP 150 antibody
see all
图片
-
Immunohistochemical analysis of paraffin-embedded Rat brain, using ab96004 at 1/500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
-
All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : NCI-H929 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa -
All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/1000 dilution
Lane 1 : Mouse brain
Lane 2 : Mouse lung
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa -
All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2000 dilution
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa -
All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution
Lane 1 : U87-MG cell lysates
Lane 2 : SK-N-SH cell lysates
Lane 3 : IMR32 cell lysates
Lane 4 : SK-N-AS cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa -
Immunofluorescence analysis of methanol-fixed HeLa, using ab96004 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.
-
Anti-DCTN1/p150-glued antibody (ab96004) at 1/3000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 142 kDa
5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, using ab96004 at 1/200 dilution. Lower image costained with Hoerchst 33342.
-
Immunohistochemical analysis of paraffin-embedded ovarian cancer, using ab96004 at 1/100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab96004 尚未被引用在任何文献中。