Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (CNS)
Specification
CNS
Other product details
Incubation time
5 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1X PBS +0.1% tritonX
Dilution
1/2000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: alexa fluor 555 goat anti rabbit IgG(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
the antibody gave a lot of background and we could not obtain clear signals to distinguish when trying to see if Dicer2 was overexpressed in CNS versus the wild type following the standard IHC protocol.
the images for this particular antibody is not available with me in jpeg format, its a Z project and is a .lsm file.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Rashmi Sipani
Verified customer
提交于 Dec 10 2014