Key features and details
- Rabbit polyclonal to Dcr 2 / Dicer 2
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
产品名称Anti-Dcr 2 / Dicer 2抗体
描述兔多克隆抗体to Dcr 2 / Dicer 2
经测试应用适用于: WBmore details
种属反应性与反应: Drosophila melanogaster
Synthetic peptide corresponding to Drosophila melanogaster Dcr 2/ Dicer 2 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available as
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 188 kDa.
Other (lower) bands are also seen in the blot (see image). We are unsure as to the identity of these - they may be cleavage products of Dcr2, or unspecific bands. These bands are all, however, blocked with the immunising peptide.
相关性RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified. The Saccharomyces cerevisiae cell cycle regulator genes DCR2 and DCR1 appear to be involved in the initiation of DNA replication. It has been predicted that DCR2 might encode a protein with phosphoesterase activity.
- Dcr2 antibody
- Dicer antibody
- Dicer2 antibody
Anti-Dcr 2 / Dicer 2 antibody (ab4732) at 1 µg/ml + Drosophila embryo nuclear extract (from D. melanogaster embryos 0-12Hr) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 188 kDa
Observed band size: 171,200 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab4732 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab4732 被引用在 11 文献中.
- Ge DT et al. The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria. Mol Cell 74:982-995.e6 (2019). PubMed: 31076285
- Harrington AW et al. Drosophila melanogaster retrotransposon and inverted repeat-derived endogenous siRNAs are differentially processed in distinct cellular locations. BMC Genomics 18:304 (2017). PubMed: 28415970
- Sinha NK & Bass BL Overexpression and purification of Dicer and accessory proteins for biochemical and structural studies. Methods 126:54-65 (2017). PubMed: 28723582
- Russo J et al. Antisense Transcription of Retrotransposons in Drosophila: An Origin of Endogenous Small Interfering RNA Precursors. Genetics 202:107-21 (2016). PubMed: 26534950
- Lim SJ et al. Requirement for CRIF1 in RNA interference and Dicer-2 stability. RNA Biol 11:1171-9 (2014). PubMed: 25483042