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Immunology Adaptive Immunity T Cells CD

Anti-DC-SIGN抗体(ab5715)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (ab5715)
  • Western blot - Anti-DC-SIGN antibody (ab5715)
  • Western blot - Anti-DC-SIGN antibody (ab5715)
  • Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody (ab5715)

Key features and details

  • Rabbit polyclonal to DC-SIGN
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72] (ab245115)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-DC-SIGN抗体
    参阅全部 DC-SIGN 一抗
  • 描述

    兔多克隆抗体to DC-SIGN
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human DC-SIGN aa 384-404.
    (Peptide available as ab6091)

  • 阳性对照

    • Human placenta tissue lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • T Cell Adhesion
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human DC-SIGN protein (ab131780)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5715于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa.
说明
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa.

靶标

  • 功能

    Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
  • 组织特异性

    Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
  • 序列相似性

    Contains 1 C-type lectin domain.
  • 结构域

    The tandem repeat domain, also called neck domain, mediates oligomerization.
  • 细胞定位

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9NNX6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 30835 Human
    • Omim: 604672 Human
    • SwissProt: Q9NNX6 Human
    • Unigene: 278694 Human
    • 别名

      • C type lectin domain family 4 member L antibody
      • C-type lectin domain family 4 member L antibody
      • CD 209 antibody
      • CD209 antibody
      • CD209 antigen antibody
      • CD209 antigen-like protein A antibody
      • CD209 molecule antibody
      • CD209_HUMAN antibody
      • Cd209a antibody
      • CDSIGN antibody
      • CIRE antibody
      • CLEC4L antibody
      • DC SIGN antibody
      • DC SIGN1 antibody
      • DC-SIGN antibody
      • DC-SIGN1 antibody
      • DCSIGN antibody
      • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
      • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
      • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
      • Dengue fever, protection against, included antibody
      • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
      • HIV GP120 Binding Protein antibody
      • MGC129965 antibody
      • MGC130443 antibody
      • SIGN-R1 antibody
      • SIGNR5 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (ab5715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (ab5715)
      Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 µg/ml.
    • Western blot - Anti-DC-SIGN antibody (ab5715)
      Western blot - Anti-DC-SIGN antibody (ab5715)

      ab5715 at 0.5 µg /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL).

       

       

      ab5715 at 0.5 µg /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL).
    • Western blot - Anti-DC-SIGN antibody (ab5715)
      Western blot - Anti-DC-SIGN antibody (ab5715)
      Lane 1 : Anti-DC-SIGN antibody (ab5715) at 1 µg/ml
      Lane 2 : Anti-DC-SIGN antibody (ab5715) at 2 µg/ml

      All lanes : Human placenta

      Observed band size: 44 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody (ab5715)
      Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody (ab5715)
      Immunofluorescence of DC-SIGN in Human Lymph Node cells using ab5715 at 20 ug/ml.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab5715?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5715 被引用在 2 文献中.

    • Green KY  et al. Human norovirus targets enteroendocrine epithelial cells in the small intestine. Nat Commun 11:2759 (2020). PubMed: 32488028
    • Moutsopoulos NM  et al. Defective neutrophil recruitment in leukocyte adhesion deficiency type I disease causes local IL-17-driven inflammatory bone loss. Sci Transl Med 6:229ra40 (2014). PubMed: 24670684

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    Inquiry: I got a TON of background and absolutely no positive staining - 10 min peroxide - 10 min protein block with Biocare's Background Sniper - 10ug/ml (manufacturer's suggestion) primary in Dako antibody diluent - formalin fixed paraffin embedded AR = 98C 20min, 90C 10sec (on other occasions I have used 135C 3.5 min 90C 10sec and that didn't work either) - secondary Biocare's ready to use rabbit-HRP antibody 20 min at RT - washing 3x5min in between each step with Biocare's Automation TBS buffer -DAB

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    Thank you for contacting Abcam regarding ab5715.


    I am sorry that you have been experiencing difficulties with this antibody in IHC. I have reviewed the protocol details you provided and I would like to get some additional information.


    What type of sample are you staining (species and tissue type)? Also, did you try other dilutions of primary? If you are getting such high background, I would recommend using less antibody. How long are you incubating with the primary? Have you also tried a longer block? Less secondary antibody may also improve your results. Be sure to include a no primary control to confirm the background is not from the secondary. If possible, please include an image with your reply.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Jul 19 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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