Key features and details
- Rabbit polyclonal to DC-SIGN
- Suitable for: ICC/IF, IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab5715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa.|
功能Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
组织特异性Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
序列相似性Contains 1 C-type lectin domain.
结构域The tandem repeat domain, also called neck domain, mediates oligomerization.
细胞定位Secreted and Cell membrane.
- Information by UniProt
- C type lectin domain family 4 member L antibody
- C-type lectin domain family 4 member L antibody
- CD 209 antibody
Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 µg/ml.
ab5715 at 0.5
µg /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL).
Lane 1 : Anti-DC-SIGN antibody (ab5715) at 1 µg/ml
Lane 2 : Anti-DC-SIGN antibody (ab5715) at 2 µg/ml
All lanes : Human placenta
Observed band size: 44 kDa why is the actual band size different from the predicted?
Immunofluorescence of DC-SIGN in Human Lymph Node cells using ab5715 at 20 ug/ml.
ab5715 被引用在 1 文献中.
- Moutsopoulos NM et al. Defective neutrophil recruitment in leukocyte adhesion deficiency type I disease causes local IL-17-driven inflammatory bone loss. Sci Transl Med 6:229ra40 (2014). PubMed: 24670684