重组Anti-DARPP32抗体[EP720Y] - BSA and Azide free (ab220808)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP720Y] to DARPP32 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-DARPP32抗体[EP720Y] - BSA and Azide free
参阅全部 DARPP32 一抗 -
描述
兔单克隆抗体[EP720Y] to DARPP32 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Rat brain, cerebral cortex and hippocampus tissue; Mouse brain and cerebral cortex tissue; Human breast adenocarcinoma; Human fetal brain tissue lysate; Human colon tissue; pig brain tissue.
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常规说明
ab220808 is the carrier-free version of ab40801.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP720Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220808于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Inhibitor of protein-phosphatase 1. -
序列相似性
Belongs to the protein phosphatase inhibitor 1 family. -
翻译后修饰
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 84152 Human
- Entrez Gene: 19049 Mouse
- Entrez Gene: 360616 Rat
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- SwissProt: Q60829 Mouse
- SwissProt: Q6J4I0 Rat
- Unigene: 286192 Human
see all -
别名
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40801).
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ab40801 at 1/20 immunoprecipitating DARPP32 in rat brain whole cell lysate observed at 32 KDa (lanes 1 and 2).
Lane 1 (input): Rat brain whole cell lysate 10μg
Lane 2 (+): ab40801 + Rat brain whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40801 in Rat brain whole cell lysate
For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40801).
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Immunocytochemistry/Immunofluorescence analysis of mouse brain tissue lysate labelling DARPP32 with purified ab40801 at 1/100 (3.4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was performed with Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40801).
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Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40801).
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Immunohistochemical analysis of paraffin-embedded human colon sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40801).
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (18)
ab220808 被引用在 18 文献中.
- Straccia M et al. Human t-DARPP is induced during striatal development. Neuroscience 333:320-30 (2016). WB, IHC-P ; Human . PubMed: 27475250
- Guo X et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun 7:12646 (2016). PubMed: 27561680
- Murata K et al. Mapping of Learned Odor-Induced Motivated Behaviors in the Mouse Olfactory Tubercle. J Neurosci 35:10581-99 (2015). IHC-P ; Mouse . PubMed: 26203152
- McKenna WL et al. Mutual regulation between Satb2 and Fezf2 promotes subcerebral projection neuron identity in the developing cerebral cortex. Proc Natl Acad Sci U S A 112:11702-7 (2015). IHC ; Mouse . PubMed: 26324926
- Lewitus GM et al. An adaptive role of TNFa in the regulation of striatal synapses. J Neurosci 34:6146-55 (2014). PubMed: 24790185