概述

  • 产品名称
  • 描述
    兔多克隆抗体to DARC
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A 16 amino acid peptide from near the carboxy terminus of human DARC.

  • 阳性对照
    • WB: Mouse brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab58965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

靶标

  • 功能
    Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
  • 组织特异性
    Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
  • 序列相似性
    Belongs to the G-protein coupled receptor Duffy family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACKR1 antibody
    • Atypical chemokine receptor 1 antibody
    • CCBP1 antibody
    • CD234 antibody
    • DARC antibody
    • Dfy antibody
    • Duffy antigen/chemokine receptor antibody
    • duffy blood group antibody
    • Duffy blood group antigen antibody
    • Duffy blood group chemokine receptor antibody
    • DUFFY_HUMAN antibody
    • FY antibody
    • Fy glycoprotein antibody
    • Glycoprotein D antibody
    • GPD antibody
    • GpFy antibody
    • Plasmodium vivax receptor antibody
    • WBCQ1 antibody
    see all

图片

  • Lane 1 : Anti-DARC antibody (ab58965) at 0.5 µg/ml
    Lane 2 : Anti-DARC antibody (ab58965) at 1 µg/ml
    Lane 3 : Anti-DARC antibody (ab58965) at 2 µg/ml

    All lanes : mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Lanes 1-2 : Anti-rabbit IgG HRP at 1/1000 dilution
    Lane 3 : Anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab58965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Samaniego R  et al. Mesenchymal contribution to recruitment, infiltration, and positioning of leukocytes in human melanoma tissues. J Invest Dermatol 133:2255-64 (2013). Human . Read more (PubMed: 23446986) »
  • Wessels JM  et al. Expression of chemokine decoy receptors and their ligands at the porcine maternal-fetal interface. Immunol Cell Biol : (2010). Pig . Read more (PubMed: 20680026) »
See all 2 Publications for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Answer

The immunogen sequence for ab40821 does not include any cysteine or methionine, so there will not be any thiol formation or methionine oxidation to affect the epitopes the antibody recognizes. The immunogen for ab58965 is near the C terminus but we do not know the exact sequence, as it is considered proprietary by the laboratory that produces the antibody.

Read More

Question
Answer

DISCOUNT CODE: ***
Expiration date: October 19th, 2012

I am very pleased to hear you would like to accept our offer and test ab58965 in IHC-P. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed on direct purchases from Abcam in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.If you will be ordering through our Canadian distributor Cedarlane, please let me know and I will be happy to issue you a different discount code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you very much for your inquiry. However, the peptide sequences used to produce this antibody is proprietary information and cannot be disclosed at this time. I am sorry that I cannot help you further in this matter but please let me know if there is anything else I can help you wit

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册