Anti-DAP3抗体(ab227257)
Key features and details
- Rabbit polyclonal to DAP3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DAP3抗体
参阅全部 DAP3 一抗 -
描述
兔多克隆抗体to DAP3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human DAP3 (internal sequence). The exact sequence is proprietary.
Database link: P51398 -
阳性对照
- IHC-P: MDA-MB-231 xenograft tissue. WB: HepG2 whole cell lysate. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227257于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Involved in mediating interferon-gamma-induced cell death. -
组织特异性
Ubiquitous. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 7818 Human
- Entrez Gene: 65111 Mouse
- Omim: 602074 Human
- SwissProt: P51398 Human
- SwissProt: Q9ER88 Mouse
- Unigene: 516746 Human
- Unigene: 29028 Mouse
- Unigene: 400397 Mouse
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别名
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
- bMRP10 antibody
see all
图片
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Anti-DAP3 antibody (ab227257) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DAP3 (green) using ab227257 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
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Paraffin-embedded MDA-MB-231 xenograft tissue stained for DAP3 with ab227257 at 1/250 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227257 尚未被引用在任何文献中。