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Kits/ Lysates/ Other Tools and Reagents IHC Tools/ Reagents

DAB Enhancer (ab675)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

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  • 数据表
  • 参考文献 (2)
  • 实验方案

概述

  • 产品名称

    DAB Enhancer
  • 常规说明

    To use as DAB signal enhancer in conjunction with peroxidase based immunostaining systems.

    i) Add one drop (50µL) of DAB enhancer in 1mL of DAB incubation buffer.
    ii) Add DAB chromogen and Peroxidase Block (3% Hydrogen Peroxide) to the above buffer.
    iii) Incubate tissue sections with the incubation medium.


    DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems.

性能

  • 形式

    Liquid
  • 存放说明

    Store at room temperature.
  • Concentration information loading...
  • Reagent说明

    DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems.
  • 研究领域

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • DAB Substrate

数据表及文件

    • Datasheet
    • SDS
  • 文献

    This product has been referenced in:

    • Zhang Z  et al. LncRNA BC032020 suppresses the survival of human pancreatic ductal adenocarcinoma cells by targeting ZNF451. Int J Oncol 52:1224-1234 (2018). Read more (PubMed: 29532883) »
    • Fernandez S  et al. Nasal immunity to staphylococcal toxic shock is controlled by the nasopharynx-associated lymphoid tissue. Clin Vaccine Immunol 18:667-75 (2011). Read more (PubMed: 21325486) »
    See all 2 Publications for this product

    发表研究结果有使用 ab675?请让我们知道,以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Thank you for your prompt response. Do you recommend any buffer to go with the heat mediated retrieval, citrate, Tris/EDTA? DAB detection system would suit, I see you have detection kits such as https://www.abcam.com/Mouse-and-Rabbit-Specific-HRPDAB-ABC-detection-IHC-kit-ab64264.html that include secondary antibody.

    I will performing immunohistochemisty using DSG3 (3G133), p63 (4A4) and RTNK2 (2C2) and if possible would like a detection kit to suit all. Would https://www.abcam.com/Mouse-and-Rabbit-Specific-HRPDAB-ABC-detection-IHC-kit-ab64264.html be suitable for this?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 06 2012

    Answer

    Thank you for contacting us.
    The kit will work with the rabbit and mouse primary antibodies.
    You can try citrate buffer pH for a start and Tris/EDTA in case the staining does not work. Please check the following for protocol and troubleshooting of;
    https://www.abcam.com/index.html?pageconfig=resource&rid=11384
    https://www.abcam.com/index.html?pageconfig=resource&rid=11467
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Dec 06 2012

    Question

    I am enquiring about Rhotekin 2 primary antibody for use with IHC-P. I see you have an antibody clone 2C2, can you provide any information on appropriate epitope retrieval methods and detection kits or secondary antibody that would be suitable for use with this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2012

    Answer

    Thank you for contacting us.
    Heat mediated antigen retrieval method with microwave or pressure cooker is recommended for this ab.
    The suitable detection system depends on the substrate you want to use. e.g. with DAB or AEC, we have following products;
    https://www.abcam.com/DAB-Enhancer-ab675.html
    https://www.abcam.com/DAB-Substrate-Kit-ab64238.html
    https://www.abcam.com/AEC-Substrate-System-Ready-to-Use-ab64252.html
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Dec 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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