为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Microbiology Organism Virus DNA Virus double stranded DNA Virus CMV

Anti-Cytomegalovirus pp28抗体[5C3] (ab6502)

  • Datasheet
Submit a review Q&A (2)References (11)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
  • Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

Key features and details

  • Mouse monoclonal [5C3] to Cytomegalovirus pp28
  • Suitable for: WB, ELISA, ICC/IF
  • Isotype: IgG2a

你可能会感兴趣

一抗
Product image
Anti-Vinculin antibody (ab155120)
蛋白
Product image
Recombinant Human HLA-DR protein (ab177661)
二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

查看更多相关产品

概述

  • 产品名称

    Anti-Cytomegalovirus pp28抗体[5C3]
  • 描述

    小鼠单克隆抗体[5C3] to Cytomegalovirus pp28
  • 宿主

    Mouse
  • 特异性

    Cytomegalovirus pp28 tegument protein. Slight cross reactivity with HSV1.

  • 经测试应用

    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性

    与反应: Other species
  • 免疫原

    Tissue, cells or virus corresponding to Cytomegalovirus pp28.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    > 95% IgG by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    5C3
  • 骨髓瘤

    NS1/1-Ag4-1
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • CMV

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Cytomegalovirus pp28 protein (ab43038)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6502于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa).
ELISA
1/100000.
ICC/IF
Use at an assay dependent concentration. PubMed: 20679731
说明
WB
1/16000. Detects a band of approximately . kDa (predicted molecular weight: 28 kDa).
ELISA
1/100000.
ICC/IF
Use at an assay dependent concentration. PubMed: 20679731

靶标

  • 相关性

    Cytomegalovirus (CMV), is a genus of Herpes viruses; in humans the species is known as Human herpesvirus 5 (HHV-5). It belongs to the Betaherpesvirinae subfamily of Herpesviridae. The name means "very big cell virus".
  • 数据库链接

    • SwissProt: P52368 Other species
    • 别名

      • 28 kDa structural phosphoprotein antibody
      • CMV pp28 tegument protein antibody
      • CMV tegument protein UL99 antibody
      • Cytomegalovirus pp28 tegument protein antibody
      • Cytomegalovirus tegument protein UL99 antibody
      • HCMV antibody
      • HHV 5 antibody
      • Human Herpesvirus 5 antibody
      • Tegument protein UL99 antibody
      • UL 99 antibody
      • UL99 antibody
      see all

    图片

    • ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
      ELISA - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

      Please see protocols tab for procedure. Antigen is CMV infected cell extract coated at a dilution of 1/100.

    • Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)
      Western blot - Anti-Cytomegalovirus pp28 antibody [5C3] (ab6502)

      See protocols tab for procedure. Antigen is CMV infected cell extract at 10 µg/cm.

    实验方案

    • Immunofluorescence
    • ELISA
    • Western blot

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (11)

    发表研究结果有使用 ab6502?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6502 被引用在 11 文献中.

    • Ghassabian H  et al. Divide et impera: An In Silico Screening Targeting HCMV ppUL44 Processivity Factor Homodimerization Identifies Small Molecules Inhibiting Viral Replication. Viruses 13:N/A (2021). PubMed: 34065234
    • Sloan E  et al. MORC3, a Component of PML Nuclear Bodies, Has a Role in Restricting Herpes Simplex Virus 1 and Human Cytomegalovirus. J Virol 90:8621-33 (2016). PubMed: 27440897
    • Lu Y  et al. Stimulation of the Replication of ICP0-Null Mutant Herpes Simplex Virus 1 and pp71-Deficient Human Cytomegalovirus by Epstein-Barr Virus Tegument Protein BNRF1. J Virol 90:9664-9673 (2016). WB . PubMed: 27535048
    • Lu Y & Everett RD Analysis of the functional interchange between the IE1 and pp71 proteins of human cytomegalovirus and ICP0 of herpes simplex virus 1. J Virol 89:3062-75 (2015). PubMed: 25552717
    • McKinney C  et al. A new role for the cellular PABP repressor Paip2 as an innate restriction factor capable of limiting productive cytomegalovirus replication. Genes Dev 27:1809-20 (2013). PubMed: 23964095
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    thaks for your answer,i'd like to know too about antibody ab6502, does it recognizes pp28 protein form HCMV or from MCMV? dou you have it in stock? waht is the price? thaks again

    Read More

    Abcam community

    Verified customer

    Asked on Feb 26 2007

    Answer

    Thank you for your enquiry. I can confirm that this antibody (ab6502) has been successfully tested for detection of human CMV pp28. It would also be predicted to detect murine CMV pp28, although this has not been tested and cannot be guaranteed. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Feb 27 2007

    Question

    I have now tested the anti-p28 antibody we recently obtained from abcam (ab6502) using the dilution and fixation conditions you suggest. The antibody really does not detect what it is meant to. On infected cells the anti-p28 antibody is intense but not specific (it does show more intense staining than an isotype matched control antibody but, as I said, it appears totally non-specific and stains all of the cell very intensely). Perhaps worse, is the extremely intense punctate nuclear fluorescence it shows on un-infected cells (which is not shown with an isotype matched control). Clearly, this antibody is not specific for HCMV infected cells and does not detect pp28 expression as detailed in your data sheet. I am happy to try another Lot number if you think there may be problems with the batch we have received. Alternatively, if you can put us in contact with anyone in the UK or Europe who has purchased this antibody, we would be happy to contact them regarding our problems. Failing that, we would appreciate a refund on the purchase cost of the antibody and be happy to send you back the antibody which we have.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 27 2004

    Answer

    Thank you for your enquiry. We are very sorry that you are dissatisfied with this particular antibody. We take your complaint very seriously and certainly we can offer you either a new vial - free of charge or a refund. Please do let us know which you would prefer. However, we would like to investigate this matter further and would highly appreciate if you could provide us a detailed protocol. This background information would help us to decide if the antibody is really specific for detecting Cytomegalovirus pp28. If not, then we will unpublish the product and withdraw it from our catalogue. 1. Please describe the problem (high background, no staining etc). 2. On what material are you testing the antibody in IHC? • Species? • Cell line? • Tissue? 3. How did you fix the samples? • Ethanol, methanol • Acetone • Paraformaldehyde • Other 4. Did you apply antigen retrieval step? • Enzymatic method • Heat mediated technique • Other 5. How did you block the unspecific binding sites? 6. Primary antibody • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 7. Secondary antibody • What secondary antibody are you using? • Specification (in which species was it raised against)? • At what dilution(s) have you tested this antibody? • Incubation, wash steps? • Do you know whether the problems you are experiencing come from the secondary? • What detection method are you using? 8. Background staining • Please provide an image of your staining 9. Which detection system did you use? 10. Did you apply positive and negative controls along with the samples? Please specify. 11. Optimization attempts • How many times have you tried the IHC? • Do you obtain the same results every time? • What steps have you altered? Thank you for your understanding and your co-operation. We are looking forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 Apr 27 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2022 艾博抗(上海)贸易有限公司版权所有