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Microbiology Organism Virus DNA Virus double stranded DNA Virus CMV

Anti-Cytomegalovirus Glycoprotein B抗体[2F12] (ab6499)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (11)

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Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
  • ELISA - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
  • Western blot - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

Key features and details

  • Mouse monoclonal [2F12] to Cytomegalovirus Glycoprotein B
  • Suitable for: WB, ELISA, ICC/IF
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Cytomegalovirus Glycoprotein B抗体[2F12]
    参阅全部 Cytomegalovirus Glycoprotein B 一抗
  • 描述

    小鼠单克隆抗体[2F12] to Cytomegalovirus Glycoprotein B
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性

    与反应: Other species
  • 免疫原

    Tissue, cells or virus corresponding to Human Cytomegalovirus Glycoprotein B. Human Cytomegalovirus (AD169) infected human fibroblast cells

  • 表位

    Epitope has not been mapped.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    2F12
  • 骨髓瘤

    NS1/1-Ag4-1
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • CMV

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Cytomegalovirus Glycoprotein B (ab43040)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6499于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
ELISA
ICC/IF
说明
应用说明
Immunofluoresence titer 1+ @ 1:12,800, IFA is tested with acetone fixtation.
ELISA titer 0.100 @ 1:102,400
Western Blot > 1:4,000

靶标

  • 相关性

    Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
  • 细胞定位

    Virus envelope.
  • 别名

    • CMV glycoprotein B antibody
    • Glycoprotein B antibody
    • HCMV antibody
    • HHV5 glycoprotein B antibody
    • UL55 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

    See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

    See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

    Determined by IFA (see protocol section) at an antibody dilution of 1:100 with anti-Mouse-FITC F(ab')2 w/ counterstain.

  • ELISA - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
    ELISA - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

    See protocol for procedure. Antigen is CMV Infected Cell Extract coated at a dilution of 1:100.

  • Western blot - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
    Western blot - Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

    See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

    See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

实验方案

  • Immunofluorescence Assay (IFA)
  • Indirect ELISA
  • Western blot for Viruses

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (11)

发表研究结果有使用 ab6499?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6499 被引用在 11 文献中.

  • Turner DL  et al. The host exosome pathway underpins biogenesis of the human cytomegalovirus virion. Elife 9:N/A (2020). PubMed: 32910773
  • van Senten JR  et al. Human Cytomegalovirus-Encoded G Protein-Coupled Receptor UL33 Facilitates Virus Dissemination via the Extracellular and Cell-to-Cell Route. Viruses 12:N/A (2020). PubMed: 32486172
  • Alba-Patiño A  et al. Nanoparticle Reservoirs for Paper-Only Immunosensors. ACS Sens 5:147-153 (2020). PubMed: 31850749
  • Pires F  et al. A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification. J Pharm Biomed Anal 156:372-378 (2018). PubMed: 29758495
  • Shimizu D  et al. Infection of endotheliotropic human cytomegalovirus of trabecular meshwork cells. Jpn J Ophthalmol 62:667-676 (2018). PubMed: 30191418
View all Publications for this product

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Cytomegalovirus Glycoprotein B antibody [2F12]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (AD169-infected HFFF)
Loading amount
100000 cells
Specification
AD169-infected HFFF
Treatment
mock/endoH/PNGaseF for 16H
Gel Running Conditions
Reduced Denaturing
Blocking step
Superblock Buffer (Pierce) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 Feb 12 2008

Question

Looking for a neutralization protocol

Read More

Abcam community

Verified customer

Asked on Mar 02 2016

Answer

Antibody ab54023 has been tested in a neutralization assay that is described in the following reference. Figure 4 shows the data for this clone.

Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014).

We do not test this antibody in a neutralization assay in-house so I do not have a protocol to send to you. A much simpler assay then the one used in the Kirchmeier paper is briefly described in the following paper. The assay involves counting PFU after incubation of virus with HCMV gB antiserum. The assay is described in the last paragraph of the paper. It does not mention applying this test to this clone or other monoclonal antibodies but it seems like an easy approach.

EMBO J. 1986 Nov; 5(11): 3057–3063.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167261/?page=1

The other HCMV antibody you have, ab6499, has not, to our knowledge, been used in a neutralization assay.

Read More

Tom Ruyle

Abcam Scientific Support

回复于 Mar 02 2016

Question

Dear Sir/Madame,
I'm contacting you concerning the following product:
Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)
Do you have any experience or knoxledge on paraffine sections immunohistochemistry for this antibody?
And how long would it take to get it in Belgium?
Thank you in advance for your help,
best regards,

Read More

Abcam community

Verified customer

Asked on Dec 04 2012

Answer

Thank you for contacting us.

Unfortunately, the Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499), this antibody has not as yet been used in staining paraffin embedded sections to our knowledge. We do not have any other antibodies against this target which have been used in this application. However, this does not mean that it would not work, simply that we have not tried it and would therefore not be able to guarantee its performance in this application.

If you would like to purchase ab6499 and use it with your tissue samples, you would be eligible to participate in the Abtrial scheme. This offer means that if you purchase ab6499 as normal, test the antibody with your samples in IHC-P and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab6499 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab6499.

If ordered tomorrow you can expect to recieve the product by Thursday the 6th of December.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Abcam Scientific Support

回复于 Dec 04 2012

Question

about the image you showed of immunofluorescence of cell infected with
hcmv and stained with antibody Anti-Cytomegalovirus Glycoprotein B
antibody [2F12]
(ab6499)(https://www.abcam.com/Cytomegalovirus-Glycoprotein-B-antibody-2F12-ab6499.html#ab6499_1.jpg),
i would know the cell type and after how many hours of infection , the
cell habe been stained.

Read More

Abcam community

Verified customer

Asked on Nov 27 2012

Answer

Thank you for contacting us.

The cells are human fibroblast infected with CMV (AD169) - acetone fixed after 48 hours of infection

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

回复于 Nov 27 2012

Question

I need an antibody to use in immunofluorescence studies on MRC-5 cells infected with Rhesus Cytomegalovirus. I have already tried with MAbs to the HCMV immediate early gene without success. As between HCMV gB and RhCMV gB 64% of homologies have been found in previous studies, now I am looking for an antibody to HCMV gB cross-reacting with RhCMV gB, do you know anything about it? Does your antibody recognize gB carboxy terminus or not ? Thank you in advance for your kind attention Yours Letizia Ceglie

Read More

Abcam community

Verified customer

Asked on Sep 06 2001

Answer

This antibody has never been tested against Rhesus CMV and 64% homology does not sound very promising - you would be better off with a polyclonal antibody. We are in the final stages of characterizing a chicken anti-CMV that may be applicable - will be available in the next month or so. If you need the single specificity (and has access to recombinant gB) perhaps the chicken polyclonal could be made monospecific via immunoaffinity purification.

Read More

Neil Hayward

Abcam Scientific Support

回复于 Sep 06 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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