细胞因子阵列-小鼠Cytokine抗体阵列(Membrane,32 Targets) (ab133994)
概述
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产品名称
细胞因子阵列-小鼠Cytokine抗体阵列(Membrane,32 Targets)
参阅全部 Cytokine Antibody Array 抗体芯片 -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
检测类型
Semi-quantitative -
种属反应性
与反应: Mouse -
产品概述
ab133994 is for simultaneous detection of 32 Mouse Cytokines. Suitable for all sample types.
Targets: 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40/p70, IL-12 p70, IL-13, IL-17, IFN-gamma, KC/CXCL1, Leptin/OB, MCP-1, MCP-5, MIP-1alpha, MIP-2, MIP-3beta, RANTES, SCF, sTNF RI, TARC, TIMP-1, TNF-alpha, Thrombopoietin, VEGF-A
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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说明
If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Multiplex Protein Detectionmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 2 vials Cytokine Antibody Array Membranes 4 units 8 units 2 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133994于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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说明 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
图片
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The cytokine antibody array was used to detect changes in cytokine/chemokine secretion by cultured mouse eosinophils treated with recombinant protein of interest or with vehicle control. Briefly, mouse primary eosinophils were harvested and cultured in suspension, 300ng/ml recombinant protein X (our protein of interest) or vehicle alone were added to the cells for 24 hours. Then the conditioned medium were harvested by centrifugation and added directly without dilution onto the cytokine antibody array (the array was first blocked according to manufacturer’s protocol). Manufacturer’s protocol was followed for the subsequent steps and the end result is shown in the following picture. The amount of three candidate cytokine/chemokines were identified to have changed in the treated conditioned medium: GCSF (H1 and H2), Leptin (K3 and K4), and KC (J3 and J4). The changes of Leptin and KC secretion were subsequently confirmed by ELISA in independent experiments.
Abreview rating 4/5 stars. Review from Abcam user community. Verified customers - Yi Chen, Ph.D. Research Fellow, Spiegelman Lab, Dana-Farber Cancer Institute, Department of Cancer Biology, Boston, USA. Submitted 25-Jun-15.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133994 被引用在 1 文献中.
- Xiao Y et al. Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. Cancer Cell 39:423-437.e7 (2021). PubMed: 33450198