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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-Cytokeratin抗体[MNF116] (ab756)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (8)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116] (ab756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116] (ab756)
  • Flow Cytometry - Anti-Cytokeratin antibody [MNF116] (ab756)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin antibody [MNF116] (ab756)

Key features and details

  • Mouse monoclonal [MNF116] to Cytokeratin
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Cytokeratin抗体[MNF116]
    参阅全部 Cytokeratin 一抗
  • 描述

    小鼠单克隆抗体[MNF116] to Cytokeratin
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • 免疫原

    Crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    MNF116
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

相关产品

  • Alternative Versions

    • Anti-Cytokeratin antibody [MNF116], prediluted (ab82612)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
应用 Ab评论 说明
IHC-P (1)
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
  • 组织特异性

    The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
  • 疾病相关

    Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
    Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
    Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
    Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal.
  • 序列相似性

    Belongs to the intermediate filament family.
  • 翻译后修饰

    Undergoes deimination of some arginine residues (citrullination).
  • 细胞定位

    Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Target information above from: UniProt accession P04264 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3848 Human
    • Omim: 139350 Human
    • SwissProt: P04264 Human
    • Unigene: 80828 Human
    • 别名

      • 67 kDa cytokeratin antibody
      • CK-1 antibody
      • CK1 antibody
      • Cytokeratin 1 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin 8 antibody
      • Cytokeratin-1 antibody
      • EHK antibody
      • EHK1 antibody
      • epidermolytic hyperkeratosis 1 antibody
      • EPPK antibody
      • Hair alpha protein antibody
      • K1 antibody
      • K2C1_HUMAN antibody
      • KB1 antibody
      • Keratin 1 antibody
      • keratin 1, type II antibody
      • Keratin 19 antibody
      • Keratin 8 antibody
      • Keratin antibody
      • keratin, type II cytoskeletal 1 antibody
      • Keratin-1 antibody
      • Krt1 antibody
      • KRT19 antibody
      • KRT1A antibody
      • KRT8 antibody
      • KRTA antibody
      • NEPPK antibody
      • type II cytoskeletal 1 antibody
      • Type-II keratin Kb1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116] (ab756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116] (ab756)
      Formalin fixed paraffin embedded human lung carcinoma stained with Cytokeratin using ABC and AEC chromogen.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116] (ab756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin antibody [MNF116] (ab756)

      ab756 staining human tonsil by IHC-P.

    • Flow Cytometry - Anti-Cytokeratin antibody [MNF116] (ab756)
      Flow Cytometry - Anti-Cytokeratin antibody [MNF116] (ab756)
      Overlay histogram showing MCF7 cells stained with ab756 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab756, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin antibody [MNF116] (ab756)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin antibody [MNF116] (ab756)

      This image was produced with ab82612, which is the same as ab756 except for the buffer. 

      ICC/IF image of ab82612 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82612, neat) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab756?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab756 被引用在 8 文献中.

    • Mallery SR  et al. Fenretinide, Tocilizumab, and Reparixin Provide Multifaceted Disruption of Oral Squamous Cell Carcinoma Stem Cell Properties: Implications for Tertiary Chemoprevention. Mol Cancer Ther 18:2308-2320 (2019). PubMed: 31515297
    • Su S  et al. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer. Cell Res 27:461-482 (2017). IHC ; Human . PubMed: 28290464
    • Bansal D  et al. Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarianSchmidtea mediterranea. Development 144:3066-3079 (2017). PubMed: 28807897
    • Di Russo J  et al. Endothelial basement membrane laminin 511 is essential for shear stress response. EMBO J 36:183-201 (2017). PubMed: 27940654
    • Arce-Cerezo A  et al. HMGA1 overexpression in adipose tissue impairs adipogenesis and prevents diet-induced obesity and insulin resistance. Sci Rep 5:14487 (2015). Mouse . PubMed: 26411793
    • Su S  et al. A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis. Cancer Cell 25:605-20 (2014). PubMed: 24823638
    • Gryspeerdt AC  et al. Differences in replication kinetics and cell tropism between neurovirulent and non-neurovirulent EHV1 strains during the acute phase of infection in horses. Vet Microbiol 142:242-53 (2010). IHC-Fr ; Human . PubMed: 19926232
    • Norton AJ  et al. Cytokeratin-specific monoclonal antibodies are reactive with tumours of smooth muscle derivation. An immunocytochemical and biochemical study using antibodies to intermediate filament cytoskeletal proteins. Histopathology 11:487-99 (1987). PubMed: 2440791

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin antibody [MNF116]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (skin)
    Permeabilization
    Yes - 0.4% Triton-X
    Specification
    skin
    Blocking step
    IHC/ICC blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 07 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin antibody [MNF116]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Sample
    Human Tissue sections (Kidney)
    Specification
    Kidney
    Permeabilization
    Yes - Wash Buffer from Dako with tween
    Fixative
    Paraformaldehyde
    Read More

    Mr. Rudolf Jung

    Verified customer

    提交于 Dec 16 2014

    Question

    Dako reports, for this same clone, reactivity with CK 5,6,8,17,19. Do you confirm reactivity with CK18?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 30 2010

    Answer

    The information should come from the following publication from 1996: Victor G. Prieto, Jorge Lugo, N. Scott McNutt Intermediate- and low-molecular-weight keratin detection with the monoclonal antibody MNF116. An immunohistochemical study on 232 paraffin-embedded cutaneous lesions Unfortunately, we have not tested cross-reactivity with CK18. I was unable to find any information on the website either for this clone.

    Read More

    Abcam Scientific Support

    回复于 Sep 30 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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