Anti-Cytokeratin 8+18抗体[5D3]，prediluted (ab847)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)](https://www.abcam.cn/ps/products/0/ab847/Images/ab847-2-anti-cytokeratin-8-18-antibody-5d37-immunohistochemistry-skin-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)")
Key features and details
- Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Cytokeratin 8+18抗体[5D3]，prediluted
参阅全部 Cytokeratin 8+18 一抗 -
描述
小鼠单克隆抗体[5D3] to Cytokeratin 8+18，prediluted -
宿主
Mouse -
特异性
This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). This antibody stains simple and glandular epithelium. This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Cytokeratin 8+18. Human breast carcinoma cell line MCF-7.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading... -
克隆
单克隆 -
克隆编号
5D3 -
骨髓瘤
unknown -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. |
| 说明 |
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IHC-P
Use at an assay dependent concentration. This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. |
靶标
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相关性
Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 3856 Human
- Entrez Gene: 3875 Human
- Omim: 148060 Human
- Omim: 148070 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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别名
- CARD2 antibody
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)Formalin-fixed, paraffin-embeded human skin tissue stained for Cytokeratin 8 +18 using ab847 in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)](https://www.abcam.cn/ps/products/0/ab847/Images/ab847-255298-anti-cytokeratin-818-antibody-5d3-prediluted-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)Immunohistochemical analysis of formalin-fixed paraffin-embedded human skin tissue sections, labelling Cytokeratin 8/18 with ab847.
数据表及文件
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Datasheet download
文献 (0)
ab847 尚未被引用在任何文献中。
