Anti-Cytokeratin 8+18抗体[5D3]，prediluted (ab847)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)

    Key features and details

    • Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Cytokeratin 8+18抗体[5D3]，prediluted
      参阅全部 Cytokeratin 8+18 一抗

    • 描述

      小鼠单克隆抗体[5D3] to Cytokeratin 8+18，prediluted

    • 宿主

      Mouse

    • 特异性

      This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). This antibody stains simple and glandular epithelium. This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Human breast carcinoma cell line MCF-7.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab847 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P Use at an assay dependent concentration.

    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.

    靶标

    • 相关性

      Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

    • 细胞定位

      Cytoplasmic

    • 数据库链接

      • 别名

        • CARD2 antibody
        • Cell proliferation inducing gene 46 protein antibody
        • Cell proliferation inducing protein 46 antibody
        • CK 8 antibody
        • CK-8 antibody
        • CK18 antibody
        • CK8 antibody
        • CYK18 antibody
        • CYK8 antibody
        • Cytokeratin 18 antibody
        • Cytokeratin 8 antibody
        • Cytokeratin-8 antibody
        • K18 antibody
        • K2C8 antibody
        • K2C8_HUMAN antibody
        • K8 antibody
        • Keratin 18 antibody
        • Keratin 8 antibody
        • Keratin antibody
        • Keratin type I cytoskeletal 18 antibody
        • keratin type II cytoskeletal 8 antibody
        • Keratin-8 antibody
        • KO antibody
        • KRT18 antibody
        • KRT8 antibody
        • type II cytoskeletal 8 antibody
        • Type-II keratin Kb8 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)

        Immunohistochemical analysis of formalin-fixed paraffin-embedded human skin tissue sections, labelling Cytokeratin 8/18 with ab847.

      文献 (0)

      发表研究结果有使用 ab847？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab847 尚未被引用在任何文献中。

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