重组HRP Anti-Cytokeratin 8抗体[EP1628Y] (ab193094)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1628Y] to Cytokeratin 8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-Cytokeratin 8抗体[EP1628Y]
参阅全部 Cytokeratin 8 一抗 -
描述
HRP兔单克隆抗体[EP1628Y] to Cytokeratin 8 -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431 cell lysate. IHC-P: FFPE human breast adenocarcinoma tissue sections.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
解离常数(KD)
KD = 4.60 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1628Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)
- Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
- PE Anti-Cytokeratin 8 antibody [EP1628Y] (ab209297)
- Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (ab210139)
- Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free (ab217173)
- Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193094于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
1/500.
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说明 |
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WB
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/500. |
靶标
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功能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
疾病相关
Cirrhosis -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- Unigene: 533782 Human
- Unigene: 708445 Human
- Unigene: 358618 Mouse
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别名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
图片
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IHC image of Cytokeratin 8 staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins, and incubated overnight at +4°C with ab193094 at a working dilution of 1 in 500. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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HRP Anti-Cytokeratin 8 antibody [EP1628Y] (ab193094) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193094 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab193094 被引用在 1 文献中.
- Kleinmanns K et al. CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model. EBioMedicine 56:102783 (2020). PubMed: 32454402