Application
IHC - Wholemount
Sample
Mouse Tissue (Mammary Gland from a WT mouse)
Specification
Mammary Gland from a WT mouse
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in CAS Block
Dilution
1/400
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa 647 Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
This antibody performs very well. The optimal antigen retrieval condition in my experience is 10mM Sodium Citrate Buffer 0.05% Tween (Tween addition is optional) pH 6.0 - An enzymatic antigen retrieval option with Proteinase K is also suitable but you need to be careful with the concentration. Too much could easily damage your tissue. The attached picture shows a mammary duct (from a WT mouse) stained for CK8 (in yellow) and Hoechst (as counterstain). The picture was taken using a Leica Microscope and using a 20x Objective.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
提交于 Oct 03 2018