Anti-Cytokeratin 8抗体[C-43] (ab2530)

Submit a review Q&A (1)References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
  • Western blot - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)

Key features and details

  • Mouse monoclonal [C-43] to Cytokeratin 8
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC

选择批间可重复性更高的重组抗体

Product image
Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Cytokeratin 8抗体[C-43]
    参阅全部 Cytokeratin 8 一抗

  • 描述

    小鼠单克隆抗体[C-43] to Cytokeratin 8

  • 宿主

    Mouse

  • 特异性

    This antibody recognises the 52.5kDa Cytokeratin 8.

  • 经测试应用

    适用于: WB, ICC/IF, IHC-P, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Sheep, Rabbit, Cow, Pig不与反应: Mouse, Rat, Chicken, Hamster, Xenopus laevis

  • 免疫原

    Tissue, cells or virus corresponding to Human Cytokeratin 8. Cytoskeleton preparation from HeLa human cervix carcinoma cell line

  • 阳性对照

    • IHC-P: Human lung FFPE tissue sections. ICC/IF: HepG2 cells WB: HeLa cell lysate Flow Cyt (Intra): MCF7 cells

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2530于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 52.5 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use a concentration of 10 µg/ml.
Flow Cyt (Intra)
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 52.5 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use a concentration of 10 µg/ml.
Flow Cyt (Intra)
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

  • 组织特异性

    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

  • 疾病相关

    Cirrhosis

  • 序列相似性

    Belongs to the intermediate filament family.

  • 翻译后修饰

    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

  • 细胞定位

    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)

    IHC image of ab2530 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2530, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
    ICC/IF image of ab2530 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2530, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
    Western blot - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
    Anti-Cytokeratin 8 antibody [C-43] (ab2530) at 2 µg/ml + HeLA cell lysate

    Predicted band size: 52.5 kDa

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)
    Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [C-43] (ab2530)

    Overlay histogram showing MCF7 cells stained with ab2530 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2530, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions. 

     

     

文献 (9)

发表研究结果有使用 ab2530？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2530 被引用在 9 文献中.

  • Kim SY  et al. Sodium butyrate inhibits high cholesterol-induced neuronal amyloidogenesis by modulating NRF2 stabilization-mediated ROS levels: involvement of NOX2 and SOD1. Cell Death Dis 11:469 (2020). PubMed: 32555166
  • Yamazaki Y  et al. PAX1 is essential for development and function of the human thymus. Sci Immunol 5:N/A (2020). PubMed: 32111619
  • Ekoue DN  et al. GPX1 Localizes to the Nucleus in Prostate Epithelium and its Levels are not Associated with Prostate Cancer Recurrence. Antioxidants (Basel) 7:N/A (2018). PubMed: 30453672
  • Canciello A  et al. Progesterone prevents epithelial-mesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties. Sci Rep 7:3761 (2017). IHC-P ; Sheep . PubMed: 28630448
  • Volpi S  et al. EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay. J Exp Med 214:623-637 (2017). PubMed: 28148688
  • Elakoum R  et al. CARM1 and PRMT1 are dysregulated in lung cancer without hierarchical features. Biochimie 97:210-8 (2014). WB ; Human . PubMed: 24211191
  • Barboni B  et al. Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation. PLoS One 8:e63256 (2013). ICC/IF ; Sheep . PubMed: 23696804
  • Impens F  et al. Mechanistic insight into taxol-induced cell death. Oncogene 27:4580-91 (2008). WB ; Human . PubMed: 18408750
  • Bártek J  et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164:215-24 (1991). PubMed: 1716305

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Question

Customer would like to know if the epitopes are known for these antibodies. She's interested in using them in ELISA.

Read More
Answer

Thank you for your phone call. Regarding ab2530, ab2531, and ab8477, the epitopes have not been determined. Once I have heard back from the originator of ab9023 I will let you know.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com