Anti-Cytokeratin 8抗体[35bH11] (ab758)
Key features and details
- Mouse monoclonal [35bH11] to Cytokeratin 8
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-Cytokeratin 8抗体[35bH11]
参阅全部 Cytokeratin 8 一抗 -
描述
小鼠单克隆抗体[35bH11] to Cytokeratin 8 -
宿主
Mouse -
Tested Applications & Species
Application Species IHC-P Human -
免疫原
Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
35bH11 -
同种型
IgM -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-P |
Human
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应用 | Ab评论 | 说明 |
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IHC-P |
说明 |
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Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
靶标
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功能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
疾病相关
Cirrhosis -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3856 Human
- Omim: 148060 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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别名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
ab758 - immunohistochemistry
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab758 被引用在 1 文献中.
- Berger R et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). PubMed: 15604246