Anti-Cytokeratin 8抗体[35bH11] (ab758)

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Key features and details

  • Mouse monoclonal [35bH11] to Cytokeratin 8
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgM

概述

  • 产品名称

    Anti-Cytokeratin 8抗体[35bH11]
    参阅全部 Cytokeratin 8 一抗

  • 描述

    小鼠单克隆抗体[35bH11] to Cytokeratin 8

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.

    • 靶标

    • 功能

      Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

    • 组织特异性

      Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

    • 疾病相关

      Cirrhosis

    • 序列相似性

      Belongs to the intermediate filament family.

    • 翻译后修饰

      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

    • 细胞定位

      Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CARD2 antibody
        • CK 8 antibody
        • CK-8 antibody
        • CK8 antibody
        • CYK8 antibody
        • CYKER antibody
        • Cytokeratin endo A antibody
        • Cytokeratin-8 antibody
        • DreK8 antibody
        • EndoA antibody
        • K2C8 antibody
        • K2C8_HUMAN antibody
        • K8 antibody
        • Keratin 8 antibody
        • Keratin type II cytoskeletal 8 antibody
        • Keratin, type II cytoskeletal 8 antibody
        • Keratin-8 antibody
        • KO antibody
        • Krt 2.8 antibody
        • KRT8 antibody
        • MGC118110 antibody
        • MGC174782 antibody
        • MGC53564 antibody
        • MGC85764 antibody
        • sb:cb186 antibody
        • Type-II keratin Kb8 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)

        ab758 - immunohistochemistry

        Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.

      文献 (1)

      发表研究结果有使用 ab758？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab758 被引用在 1 文献中.

      • Berger R  et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). PubMed: 15604246

      客户评价及客户问答

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      Question


      Inquiry: I'm looking into a panel of antibodies from your company to use in cells of pig origin. ab758 ab27988 ab7504 ab124687 ab28028 ab11333 ab32362 I would like to know the following: Have they been tested in pig cells? If yes, are they reactive towards pig cells? If you do not have that information, can you tell me the epitope or peptide sequence that was used in generating that antibody? If you do not want to tell me that info, can you tell me the identity percentage of match of the pig compared to the epitope that it was used in generating the antibody. Many thanks! S

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      Answer

      Thank you for your inquiry.

      For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig cytokeratin 8 sequence. We generally say anything with >85% homology would be expected to cross-react, so this is right on the edge. However, we do have two anti-cytokeratin 8 antibodies that we know do work in pig as ab2531 and ab2530.

      https://www.abcam.com/Cytokeratin-8-antibody-C-51-ab2531.html

      https://www.abcam.com/Cytokeratin-8-antibody-C-43-ab2530.html

      For ab27988, this antibody has been validated in pig and we therefore guarantee it will work for pig in IHC-P, IHC-Fr in accordance with our Abpromise.

      For ab7504, the immunogen was human breast carcinoma cell line, MCF-7. Pig and human EpCAM is 83% homologous, so this is a little low. We have an anti-EpCAM that is predicted (>90% homology) to cross-react with pig, and that is ab71916. However, this hasn't been validated and therefore not guaranteed in pig.

      https://www.abcam.com/EpCAM-antibody-ab71916.html

      I have to check with the lab on ab32362 and ab124687 since I don't have the immunogens readily available to BLAST.

      For ab28028, full length native protein (purified): Vimentin isolated from bovine eye lens was used as an immunogen. Pig vimentin and bovine vimentin are 97% homologous, so there is a good chance this will cross-react with pig. However this hasn't been tested and therefore isn't validated in pig. We do have a few vimentin antibodies that work in pig though,like ab20346 and ab8069.

      https://www.abcam.com/Vimentin-antibody-VI-10-ab20346.html

      https://www.abcam.com/Vimentin-antibody-V9-ab8069.html

      For ab11333, cultured human thymic fibroblasts was used as an immunogen. We don't have a particular protein sequence to look up for this one, so unfortunately we don't know how/if it will work with pig.

      I hope this information helps. I'll be in touch again once I hear about ab32362 and ab124687.

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