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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-Cytokeratin 8抗体[35bH11] (ab758)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)

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  • 数据表
  • 参考文献 (1)
  • 实验方案

概述

  • 产品名称

    Anti-Cytokeratin 8抗体[35bH11]
    参阅全部 Cytokeratin 8 一抗
  • 描述

    小鼠单克隆抗体[35bH11] to Cytokeratin 8
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, IHC-Frmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    35bH11
  • 同种型

    IgM
  • 轻链类型

    unknown
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)
  • Recombinant Protein

    • Recombinant Human Cytokeratin 8 protein (ab156970)

应用

Our Abpromise guarantee covers the use of ab758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IHC-Fr
  • 应用说明
    IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
  • 靶标

    • 功能

      Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 组织特异性

      Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 疾病相关

      Cirrhosis
    • 序列相似性

      Belongs to the intermediate filament family.
    • 翻译后修饰

      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 细胞定位

      Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Target information above from: UniProt accession P05787 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3856 Human
      • Omim: 148060 Human
      • SwissProt: P05787 Human
      • Unigene: 533782 Human
      • Unigene: 708445 Human
      • 别名

        • CARD2 antibody
        • CK 8 antibody
        • CK-8 antibody
        • CK8 antibody
        • CYK8 antibody
        • CYKER antibody
        • Cytokeratin endo A antibody
        • Cytokeratin-8 antibody
        • DreK8 antibody
        • EndoA antibody
        • K2C8 antibody
        • K2C8_HUMAN antibody
        • K8 antibody
        • Keratin 8 antibody
        • Keratin type II cytoskeletal 8 antibody
        • Keratin, type II cytoskeletal 8 antibody
        • Keratin-8 antibody
        • KO antibody
        • Krt 2.8 antibody
        • KRT8 antibody
        • MGC118110 antibody
        • MGC174782 antibody
        • MGC53564 antibody
        • MGC85764 antibody
        • sb:cb186 antibody
        • Type-II keratin Kb8 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)

        ab758 - immunohistochemistry

        Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.

      实验方案

      • Immunohistochemistry protocols

      数据表及文件

      • Datasheet
      • SDS
    • 文献

      This product has been referenced in:

      • Berger R  et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »
      See 1 Publication for this product

      发表研究结果有使用 ab758?请让我们知道,以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

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      提交评价 提交问题

      Question


      Inquiry: I'm looking into a panel of antibodies from your company to use in cells of pig origin. ab758 ab27988 ab7504 ab124687 ab28028 ab11333 ab32362 I would like to know the following: Have they been tested in pig cells? If yes, are they reactive towards pig cells? If you do not have that information, can you tell me the epitope or peptide sequence that was used in generating that antibody? If you do not want to tell me that info, can you tell me the identity percentage of match of the pig compared to the epitope that it was used in generating the antibody. Many thanks! S

      Read More

      Abcam community

      Verified customer

      Asked on May 09 2012

      Answer

      Thank you for your inquiry.

      For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig cytokeratin 8 sequence. We generally say anything with >85% homology would be expected to cross-react, so this is right on the edge. However, we do have two anti-cytokeratin 8 antibodies that we know do work in pig as ab2531 and ab2530.

      https://www.abcam.com/Cytokeratin-8-antibody-C-51-ab2531.html

      https://www.abcam.com/Cytokeratin-8-antibody-C-43-ab2530.html

      For ab27988, this antibody has been validated in pig and we therefore guarantee it will work for pig in IHC-P, IHC-Fr in accordance with our Abpromise.

      For ab7504, the immunogen was human breast carcinoma cell line, MCF-7. Pig and human EpCAM is 83% homologous, so this is a little low. We have an anti-EpCAM that is predicted (>90% homology) to cross-react with pig, and that is ab71916. However, this hasn't been validated and therefore not guaranteed in pig.

      https://www.abcam.com/EpCAM-antibody-ab71916.html

      I have to check with the lab on ab32362 and ab124687 since I don't have the immunogens readily available to BLAST.

      For ab28028, full length native protein (purified): Vimentin isolated from bovine eye lens was used as an immunogen. Pig vimentin and bovine vimentin are 97% homologous, so there is a good chance this will cross-react with pig. However this hasn't been tested and therefore isn't validated in pig. We do have a few vimentin antibodies that work in pig though,like ab20346 and ab8069.

      https://www.abcam.com/Vimentin-antibody-VI-10-ab20346.html

      https://www.abcam.com/Vimentin-antibody-V9-ab8069.html

      For ab11333, cultured human thymic fibroblasts was used as an immunogen. We don't have a particular protein sequence to look up for this one, so unfortunately we don't know how/if it will work with pig.

      I hope this information helps. I'll be in touch again once I hear about ab32362 and ab124687.

      Read More

      Abcam Scientific Support

      回复于 May 09 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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