Anti-Cytokeratin 8抗体[35bH11] (ab758)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-Cytokeratin 8抗体[35bH11]
参阅全部 Cytokeratin 8 一抗 -
描述小鼠单克隆抗体[35bH11] to Cytokeratin 8
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宿主Mouse
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经测试应用适用于: IHC-P, IHC-Frmore details
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种属反应性与反应: Human
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免疫原
Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant -
纯度Tissue culture supernatant
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克隆单克隆
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克隆编号35bH11
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同种型IgM
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轻链类型unknown
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| IHC-Fr |
应用说明
IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
靶标
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功能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
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组织特异性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
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疾病相关Cirrhosis
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序列相似性Belongs to the intermediate filament family.
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翻译后修饰Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
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数据库链接
- Entrez Gene: 3856 Human
- Omim: 148060 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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别名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)](https://www.abcam.cn/ps/products/0/ab758/Images/ab758_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)ab758 - immunohistochemistry
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)](https://www.abcam.cn/ps/products/0/ab758/Images/Cytokeratin-8-Primary-antibodies-ab758-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.
文献
This product has been referenced in:
- Berger R et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"