Anti-Cytokeratin 8抗体[35bH11] (ab758)
概述
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产品名称
Anti-Cytokeratin 8抗体[35bH11]
参阅全部 Cytokeratin 8 一抗 -
描述
小鼠单克隆抗体[35bH11] to Cytokeratin 8 -
宿主
Mouse -
经测试应用
适用于: IHC-P, IHC-Frmore details -
种属反应性
与反应: Human -
免疫原
Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
35bH11 -
同种型
IgM -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | ||
IHC-Fr |
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
靶标
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功能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
疾病相关
Cirrhosis -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
细胞定位
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3856 Human
- Omim: 148060 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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别名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
ab758 - immunohistochemistry
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.
文献
This product has been referenced in:
- Berger R et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »