重组
RabMAb

Anti-Cytokeratin 5抗体[EP1601Y] (ab52635)

概述

  • 产品名称
    Anti-Cytokeratin 5抗体[EP1601Y]
    参阅全部 Cytokeratin 5 一抗
  • 描述
    兔单克隆抗体[EP1601Y] to Cytokeratin 5
  • 宿主
    Rabbit
  • 特异性
    Mouse reactivity is based on IHC ( positive tissues: liver,lung,brain,skin). However, WB was negative for Mouse brain, heart, kidney and spleen. There is background staining in mouse and rat islet.
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Cytokeratin 5 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P13647

  • 阳性对照
    • WB: A431 cell, human fetal skin, rat skin and mouse skin lysates. IHC-P: Squamous cell cervical, squamous cell lung and basal cell breast carcinoma tissue. Human transitional urinary bladder carcinoma tissue. Normal tonsil squamous, human cervical carcinoma, mouse skin and rat skin tissues. Flow Cytometry and ICC/IF: A431 cells. ICC/IF: A431 cells.
  • 常规说明

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    EP1601Y
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
WB 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Flow Cyt 1/20.
IHC-P 1/200.

靶标

  • 疾病相关
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
    Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
  • 序列相似性
    Belongs to the intermediate filament family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 58 kDa cytokeratin antibody
    • CK-5 antibody
    • CK5 antibody
    • Cytokeratin-5 antibody
    • Cytokeratin5 antibody
    • DDD antibody
    • DDD1 antibody
    • EBS2 antibody
    • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
    • K2C5_HUMAN antibody
    • K5 antibody
    • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody
    • Keratin 5 antibody
    • Keratin antibody
    • keratin complex 2, basic, gene 5 antibody
    • keratin, type II cytoskeletal 5 antibody
    • Keratin-5 antibody
    • Keratin5 antibody
    • KRT 5 antibody
    • Krt5 antibody
    • KRT5A antibody
    • type II cytoskeletal 5 antibody
    • Type-II keratin Kb5 antibody
    see all

图片

  • All lanes : Anti-Cytokeratin 5 antibody [EP1601Y] (ab52635) at 1/10000 dilution (purified)

    Lane 1 : Human fetal skin lysates
    Lane 2 : Rat skin lysates
    Lane 3 : Mouse skin lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 6 with Purified ab52635 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Unpurified ab52635 showing positive staining in squamous cell cervical carcinoma tissue.

  • A431 cells stained with unpurified ab52635 at 1/100 - 1/250

  • Anti-Cytokeratin 5 antibody [EP1601Y] (ab52635) at 1/10000 dilution (unpurified) + A431 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Human transitional urinary bladder carcinoma stained with unpurified ab52635 at 1/100 - 1/250 dilution.

  • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Cytokeratin 5 with purified ab52635 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Unpurified ab52635 showing positive staining in basal cell breast carcinoma tissue.

  • Unpurified ab52635 showing negative staining in ductal breast carcinoma tissue.

  • Unpurified ab52635 showing negative staining in stomach adenocarcinoma tissue.

  • Unpurified ab52635 showing positive staining in normal tonsil squamous cells tissue.

  • Unpurified ab52635 showing positive staining in squamous cell lung carcinoma tissue.

文献

This product has been referenced in:
  • Schulz D  et al. Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry. Cell Syst 6:25-36.e5 (2018). Read more (PubMed: 29289569) »
  • Pellinen T  et al. ITGB1-dependent upregulation of Caveolin-1 switches TGFß signalling from tumour-suppressive to oncogenic in prostate cancer. Sci Rep 8:2338 (2018). IHC . Read more (PubMed: 29402961) »

See all 28 Publications for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (primary keratinocytes)
Gel Running Conditions
Reduced Denaturing (gel anyKd from BioRad)
Loading amount
15 µg
Specification
primary keratinocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Julien Coutier

Verified customer

提交于 May 12 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 10 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Skin
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 23 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (Airway epithelium (basal epithelial cells))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
No
Specification
Airway epithelium (basal epithelial cells)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (olfactory epithelium)
Specification
olfactory epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.03% triton x100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human breast cancer cell xenograft sections)
Specification
human breast cancer cell xenograft sections
Fixative
formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.1M Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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