重组

重组Anti-Cytokeratin 5 + 6 + 18抗体[LP34] (ab40)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [LP34] to Cytokeratin 5 + 6 + 18
  • Suitable for: WB, IHC-P
  • Reacts with: Human

概述

  • 产品名称

    Anti-Cytokeratin 5 + 6 + 18抗体[LP34]
    参阅全部 Cytokeratin 5 + 6 + 18 一抗

  • 描述

    小鼠单克隆抗体[LP34] to Cytokeratin 5 + 6 + 18

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus within Human Cytokeratin 5 + 6 + 18. The exact sequence is proprietary. P13647, P05783, P02538.
    Sequence:

    Detergent-insoluble fraction of psoriatic human epidermis

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • IHC-P: Human skin and tonsil tissue. Human pancreatic carcinoma and bladder carcinoma tissue. WB: Human skin lysate. HeLa, MCF7 and HepG2 whole cell lysate.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab40 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.424 µg/ml.
IHC-P Use a concentration of 0.085 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for ICC/IF.

    • 靶标

    • 相关性

      Keratins 5, 6 are members of type II keratins family that are specifically expressed in the inner root sheath of hair follicles. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin 18 is memeber of the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family.

    • 细胞定位

      Cytoplasmic

    • 数据库链接

      • 别名

        • CK5 antibody
        • CYK18 antibody
        • K18 antibody
        • K5 antibody
        • K6IRS2 antibody
        • Keratin 18 antibody
        • Keratin 5 antibody
        • Keratin 72 antibody
        • KRT18 antibody
        • KRT5 antibody
        • KRT6 antibody
        • KRT72 antibody
        see all

      图片

      • Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        All lanes : Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 0.424 µg/ml

        Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
        Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
        Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

        Observed band size: 45 kDa
        why is the actual band size different from the predicted?



        Blocking/Dilution buffer: 5% NFDM/TBST.

        Exposure times: Lane 1: 15 secs; Lane 2-3: 3 secs.

      • Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 1/1000 dilution + Human skin lysate at 20 µg

        Secondary
        Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

        Observed band size: 58 kDa why is the actual band size different from the predicted?


        Exposure time: 114 seconds


        Blocking/Dilution buffer: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

        Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 5 + 6 + 18 with ab40 at 1/1000 dilution, followed by a ready to use secondary. Positive staining on human skin is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

        Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on the epithelial cells of human tonsil tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

        Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human bladder carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

        Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human pancreatic carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
        Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (4)

      发表研究结果有使用 ab40？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab40 被引用在 4 文献中.

      • Barton D  et al. ELP3 localises to mitochondria and actin-rich domains at edges of HeLa cells. Neurosci Lett 455:60-4 (2009). PubMed: 19429107
      • Gardiner J  et al. Defects in tongue papillae and taste sensation indicate a problem with neurotrophic support in various neurological diseases. Neuroscientist 14:240-50 (2008). PubMed: 18270312
      • Karashima T & Watt FM Interaction of periplakin and envoplakin with intermediate filaments. J Cell Sci 115:5027-37 (2002). PubMed: 12432088
      • Braun-Falco M  et al. Efficient gene transfer into human keratinocytes with recombinant adeno-associated virus vectors. Gene Ther 6:432-41 (1999). PubMed: 10435093

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