Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer)
Loading amount
35 µg
Specification
pancreatic cancer
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: Amersham ECL-HRP
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 44 kDa Non-specific: ca.55 kDa
Additional data
Additional Notes
4 human pancreatic ductal adenocarcinoma cell lines are analyzed:
1. Panc1 2. CaPan1 3. MiaPaCa 4. Paca3
1. Panc1 2. CaPan1 3. MiaPaCa 4. Paca3
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Pawel Mazur
Verified customer
提交于 Jun 09 2010