Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Blocking step
PBS + 0.1% Triton X-100, 1% BSA, 10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% Triton X-100, 1% BSA
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Fresh-frozen cryosections post-fixed w/ PBS + 2% formaldehyde for 10 min. Stained w/ DAPI.
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提交于 Aug 03 2012