Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human breast carcinoma xenograft in mouse)
Specification
Human breast carcinoma xenograft in mouse
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: tNB
Secondary antibody
Name
Non-Abcam antibody was used: Vector
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
15µm cryostat cut sections well-fixed in PFA. Applied streptavidin-HRP after secondary and then amplified with Perkin Elmer TSA kit. Developed fluorescence with streptavidin Alexa546.
Micrograph demonstrates cytokeratin 18+ human breast tumor xenograft in a mouse (red). Blue = Hoechst
Micrograph demonstrates cytokeratin 18+ human breast tumor xenograft in a mouse (red). Blue = Hoechst
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提交于 May 01 2009