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Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-Cytokeratin 18抗体[DC 10] (ab7797)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (15)

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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
  • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
  • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

Key features and details

  • Mouse monoclonal [DC 10] to Cytokeratin 18
  • Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
Anti-Cytokeratin 18 antibody [E431-1] (ab32118)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Cytokeratin 18抗体[DC 10]
    参阅全部 Cytokeratin 18 一抗
  • 描述

    小鼠单克隆抗体[DC 10] to Cytokeratin 18
  • 宿主

    Mouse
  • 特异性

    Specific reactivity for the cytokeratin 18 (45 kD) polypeptide.
  • 经测试应用

    适用于: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details
  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig
  • 免疫原

    Tissue, cells or virus corresponding to Human Cytokeratin 18. PMC-42 human breast carcinoma cells.

  • 阳性对照

    • ICC/IF: Hep-2 cells. IHC-P: Human colon carcinoma. WB: HeLa, HT-29 cell lysate. Flow Cyt (Intra): MCF-7 cells; HeLa cells.
  • 常规说明

    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from TCS
  • Primary antibody说明

    This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
  • 克隆

    单克隆
  • 克隆编号

    DC 10
  • 同种型

    IgG1
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7797于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 10 µg/ml.
ICC/IF (6)
1/400.
Flow Cyt (Intra)
Use a concentration of 6 - 16 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (1)
Use a concentration of 1 - 2 µg/ml.
说明
IHC-P
Use a concentration of 10 µg/ml.
ICC/IF
1/400.
Flow Cyt (Intra)
Use a concentration of 6 - 16 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use a concentration of 1 - 2 µg/ml.

靶标

  • 功能

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性

    Belongs to the intermediate filament family.
  • 翻译后修饰

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位

    Cytoplasm > perinuclear region.
  • Target information above from: UniProt accession P05783 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3875 Human
    • Omim: 148070 Human
    • SwissProt: P05783 Human
    • Unigene: 406013 Human
    • 别名

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

      Immunocytochemistry staining of Cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (ab7797, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 µg/ml; orange).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

      Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml

    • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
      Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
      All lanes : Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 2 µg/ml

      Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Non-Reducing)
      Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Non-Reducing)
      Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Reducing)
      Lane 4 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Reducing)

      Secondary
      All lanes : IRDye800-conjugated anti-mouse secondary antibody.


      A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.

    • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
      Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
      Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

      Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

       

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (15)

    发表研究结果有使用 ab7797?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7797 被引用在 15 文献中.

    • Carmell N  et al. Identification and Validation of ERK5 as a DNA Damage Modulating Drug Target in Glioblastoma. Cancers (Basel) 13:N/A (2021). PubMed: 33668183
    • Han L  et al. Conditionally reprogrammed macaque endocervical cells retain steroid receptor expression and produce mucus. Biol Reprod 102:1191-1202 (2020). PubMed: 32232331
    • Al-Khalaf HH  et al. Senescent Breast Luminal Cells Promote Carcinogenesis through Interleukin-8-Dependent Activation of Stromal Fibroblasts. Mol Cell Biol 39:N/A (2019). PubMed: 30397077
    • Deng H  et al. Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. J Cell Physiol 234:7683-7694 (2019). PubMed: 30609028
    • Siefert J  et al. Hepatocyte Transplantation to the Liver via the Splenic Artery in a Juvenile Large Animal Model. Cell Transplant 28:14S-24S (2019). PubMed: 31842585
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [DC 10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human retinal pigment epithelium (ARPE-19))
    Permeabilization
    Yes - TritonX 0,3%
    Specification
    Human retinal pigment epithelium (ARPE-19)
    Blocking step
    Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 26 2015

    ELISPOT abreview for Anti-Cytokeratin 18 antibody [DC 10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    ELISpot
    Sample
    Human Recombinant protein (E coli)
    Specification
    E coli
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    Abcam user community

    Verified customer

    提交于 May 12 2015

    ELISA abreview for Anti-Cytokeratin 18 antibody [DC 10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (Human Cytokeratin 18 full length protein (ab73638))
    Specification
    Human Cytokeratin 18 full length protein (ab73638)
    Type
    Sandwich (Detection)
    Blocking step
    StartingBlock blocking buffer (Thermo Scientific) as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 25 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [DC 10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Human Cell (hIPSC-derived endocrine cells)
    Specification
    hIPSC-derived endocrine cells
    Permeabilization
    Yes - 0.1 % triton x100
    Fixative
    Formaldehyde
    Read More

    Dr. Youngjin Kim

    Verified customer

    提交于 Mar 24 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 18 antibody [DC 10]

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    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human breast carcinoma xenograft in mouse)
    Specification
    Human breast carcinoma xenograft in mouse
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 01 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [DC 10]

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    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MDA468)
    Specification
    MDA468
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 01 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [DC 10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (C4-2b (prostate cancer cell line))
    Specification
    C4-2b (prostate cancer cell line)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% triton
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 0.2% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 15 2008

    Western blot abreview for Anti-Cytokeratin 18 antibody [DC 10]

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    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (pancreatic cancer cell line)
    Loading amount
    20 µg
    Specification
    pancreatic cancer cell line
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Hannah Whiteman

    Verified customer

    提交于 Jul 04 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [DC 10]

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    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (pancreatic cancer cell line)
    Specification
    pancreatic cancer cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Dr. Hannah Whiteman

    Verified customer

    提交于 Jun 18 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [DC 10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP)
    Specification
    LNCaP
    Fixative
    Acetone
    Permeabilization
    Yes - 0.1% Triton-X
    Blocking step
    Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Dr. Vivien D'Arcy

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    提交于 Mar 06 2008

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