Anti-Cytokeratin 18抗体[C-04] (ab668)
Key features and details
- Mouse monoclonal [C-04] to Cytokeratin 18
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human, Mammals
- Isotype: IgG1
概述
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产品名称
Anti-Cytokeratin 18抗体[C-04]
参阅全部 Cytokeratin 18 一抗 -
描述
小鼠单克隆抗体[C-04] to Cytokeratin 18 -
宿主
Mouse -
特异性
Human Cytokeratin -
Tested Applications & Species
Application Species Flow Cyt Human -
免疫原
Tissue, cells or virus corresponding to Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
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阳性对照
- IHC-P: Human skin tissue. Flow Cyt: HCT 116 cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
C-04 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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Flow Cyt |
Human
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应用 | Ab评论 | 说明 |
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IHC-P | (11) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
Also see PMID 18946470. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use a concentration of 1 - 4 µg/ml. Also see PMID 18946470. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
组织特异性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
疾病相关
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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别名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
图片
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Flow cytometric pattern (intracellular staining) of HeLa cells (Human epithelial cell line from cervix adenocarcinoma cell suspension) stained with ab668 (0.6 µg/ml concentration)
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Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labelling cytokeratin-18 with ab668 at 10µg/ml concentration.
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Flow cytometric analysis (intracellular staining) of HeLa cells (human epithelial cell line from cervix adenocarcinoma cell suspension) stained with 0.6 µg/ml ab668 (in red), and unstained HeLa cells (black).
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Overlay histogram showing HCT 116 (Human colorectal carcinoma cell line) cells stained with ab668 (red line).
The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab668, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT 116 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)This image is courtesy of an Abreview submitted by Carl Hobbs
ab668 staining Cytokeratin 18 in cat lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Coloured arrowheads indicate positivity.
Good immunolabeling of bronchial tree epithelia (green), alveolar lining epithelium (red ).
Goblet cells are negative (asterisk).
Heavily stained structures are submucosal mucous glands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)This image is courtesy of an Abreview submitted by Carl Hobbs
ab668 staining cytokeratin 18 in mouse epithelium tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Composite image of d14 embryo shows developing epithelia.
Upper image: positive simple epithelium of renal tubules
Lower image: negative stratified epithelium of epidermis
实验方案
文献 (99)
ab668 被引用在 99 文献中.
- Zhao L et al. TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh. Nat Commun 11:341 (2020). PubMed: 31953403
- McCormick A et al. Placental endothelin-converting enzyme-1 is decreased in preeclampsia. Pregnancy Hypertens 20:108-110 (2020). PubMed: 32278308
- Zhang B et al. Klf5 acetylation regulates luminal differentiation of basal progenitors in prostate development and regeneration. Nat Commun 11:997 (2020). PubMed: 32081850
- Tamura R et al. XCL1 expression correlates with CD8-positive T cells infiltration and PD-L1 expression in squamous cell carcinoma arising from mature cystic teratoma of the ovary. Oncogene 39:3541-3554 (2020). PubMed: 32115573
- Cheng L et al. Properties of an alginate-gelatin-based bioink and its potential impact on cell migration, proliferation, and differentiation. Int J Biol Macromol 135:1107-1113 (2019). PubMed: 31173833