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Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-Cytokeratin 18抗体[C-04] (ab668)

  • Datasheet
  • SDS
Reviews (22)Q&A (9)References (99)

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Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)
  • Immunohistochemistry paraffin embedded sections - Anti-Cytokeratin 18 antibody [C-04] (ab668)
  • Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)
  • Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)

Key features and details

  • Mouse monoclonal [C-04] to Cytokeratin 18
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human, Mammals
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Cytokeratin 18抗体[C-04]
    参阅全部 Cytokeratin 18 一抗
  • 描述

    小鼠单克隆抗体[C-04] to Cytokeratin 18
  • 宿主

    Mouse
  • 特异性

    Human Cytokeratin
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data
  • 免疫原

    Tissue, cells or virus corresponding to Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431

  • 阳性对照

    • IHC-P: Human skin tissue. Flow Cyt: HCT 116 cells.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from culture supernatant. Purity >95% by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    C-04
  • 同种型

    IgG1
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

相关产品

  • Alternative Versions

    • Biotin Anti-Cytokeratin 18 antibody [C-04] (ab211441)
    • Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)
    • FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
应用 Ab评论 说明
IHC-P (11)
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

Also see PMID 18946470.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

Also see PMID 18946470.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性

    Belongs to the intermediate filament family.
  • 翻译后修饰

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位

    Cytoplasm > perinuclear region.
  • Target information above from: UniProt accession P05783 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3875 Human
    • Omim: 148070 Human
    • SwissProt: P05783 Human
    • Unigene: 406013 Human
    • 别名

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    图片

    • Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)
      Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)

      Flow cytometric pattern (intracellular staining) of HeLa cells (Human epithelial cell line from cervix adenocarcinoma cell suspension) stained with ab668  (0.6 µg/ml concentration)

    • Immunohistochemistry paraffin embedded sections - Anti-Cytokeratin 18 antibody [C-04] (ab668)
      Immunohistochemistry paraffin embedded sections - Anti-Cytokeratin 18 antibody [C-04] (ab668)

      Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labelling cytokeratin-18 with ab668 at 10µg/ml concentration.

    • Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)
      Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)

      Flow cytometric analysis (intracellular staining) of HeLa cells (human epithelial cell line from cervix adenocarcinoma cell suspension) stained with 0.6 µg/ml ab668 (in red), and unstained HeLa cells (black).

    • Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)
      Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)

      Overlay histogram showing HCT 116 (Human colorectal carcinoma cell line) cells stained with ab668 (red line).

      The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab668, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT 116 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)This image is courtesy of an Abreview submitted by Carl Hobbs

      ab668 staining Cytokeratin 18 in cat lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

      Coloured arrowheads indicate positivity.
      Good immunolabeling of bronchial tree epithelia (green), alveolar lining epithelium (red ).
      Goblet cells are negative (asterisk).
      Heavily stained structures are submucosal mucous glands.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)This image is courtesy of an Abreview submitted by Carl Hobbs

      ab668 staining cytokeratin 18 in mouse epithelium tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

      Composite image of d14 embryo shows developing epithelia.

      Upper image: positive simple epithelium of renal tubules

      Lower image: negative stratified epithelium of epidermis

      See Abreview

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (99)

    发表研究结果有使用 ab668?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab668 被引用在 99 文献中.

    • Zhao L  et al. TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh. Nat Commun 11:341 (2020). PubMed: 31953403
    • McCormick A  et al. Placental endothelin-converting enzyme-1 is decreased in preeclampsia. Pregnancy Hypertens 20:108-110 (2020). PubMed: 32278308
    • Zhang B  et al. Klf5 acetylation regulates luminal differentiation of basal progenitors in prostate development and regeneration. Nat Commun 11:997 (2020). PubMed: 32081850
    • Tamura R  et al. XCL1 expression correlates with CD8-positive T cells infiltration and PD-L1 expression in squamous cell carcinoma arising from mature cystic teratoma of the ovary. Oncogene 39:3541-3554 (2020). PubMed: 32115573
    • Cheng L  et al. Properties of an alginate-gelatin-based bioink and its potential impact on cell migration, proliferation, and differentiation. Int J Biol Macromol 135:1107-1113 (2019). PubMed: 31173833
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 31 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Cytokeratin 18 antibody [C-04]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Endocervical epithelial cells)
    Permeabilization
    Yes - 0.75% Triton-X
    Specification
    Endocervical epithelial cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 28 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [C-04]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (amnion epithelial cell)
    Permeabilization
    No
    Specification
    amnion epithelial cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 10 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 18 antibody [C-04]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skin, oral epithelium)
    Permeabilization
    No
    Specification
    Skin, oral epithelium
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 29 2017

    Western blot abreview for Anti-Cytokeratin 18 antibody [C-04]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (T24 cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    35 µg
    Specification
    T24 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 12 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 18 antibody [C-04]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Pig Tissue sections (Small intestine)
    Specification
    Small intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Sep 11 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 18 antibody [C-04]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Cat Tissue sections (Lung)
    Specification
    Lung
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Jan 13 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 18 antibody [C-04]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Goat Tissue sections (Lung)
    Specification
    Lung
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Jan 13 2014

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Cytokeratin 18 antibody [C-04]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1:1000 · Temperature: RT°C
    Sample
    Rabbit Tissue sections (eye)
    Specification
    eye
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Dr. Alex Zagariya

    Verified customer

    提交于 Jan 06 2014

    Question


    Thank you for your reply! I know Ab668 is a popular one, but most mouse
    antibodies have background when stain on mouse tissue.
    The Ab668 also has high background in our mouse olfactory epithelium
    tissue. Please check the attachment file. That's why I want to order a
    rabbit anti-ck18.
    Our protocol as follows:
    1. olfactory epithelium tissue fixed in 4%PFA at 4C overnight
    wash and equilibrated in sucrose/PBS, embeded in OCT.
    2. Cryosections were prepared at 10 um. 4%PFA postfixed for 5min.
    3. Washed in PBS and blocked in 10% Normal Goat serum or Donkey serum plus
    1%BSA. 4C overnight.
    4. Ms-antiCK18(Ab668) 1:100 in blocking buffer 4C 48 hours
    5. Washed in PBS 4x and incubated with Gt-anti Ms Alex fluo 546 Dilution
    1000, incubated 1.5hour at Room temperature. Then wash in PBS 4X.
    According to the publications, CK18 stain the cells on the apical layer in
    Olfactory epithelium. But Ab668 stained everywhere.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 31 2013

    Answer

    Hello! Please find below our mouse on mouse staining tips. This may help you to improve your results. Additionally I would recommend you reduce the primary incubation to overnight, and incubate the antibodies in a solution of TBS plus 1% BSA.

    We cannot vouch for the Millipore version of the clone but we have tested ab32118 and found that it does not react in mouse, and have placed this information on our datasheet to inform our customers.



    Tips for eliminating background when staining mouse tissue with a mouse monoclonal



    View a pdf version of this page
    Staining of mouse tissue using mouse antibody is a complicated process as high levels of background are often observed. It is notoriously difficult to eliminate this background.
    Much of the background is caused by secondary antibody binding to endogenous mouse IgG in the tissue being stained, and to Fc receptors on B cells, plasma cells and macrophages.
    Abcam is unable to guarantee monoclonal mouse antibodies on mouse tissue (unless stated on the datasheet). However, there are a few tips to try and reduce this background should mouse on mouse staining be necessary:

    1. Blocking of endogenous IgG



    Prepare tissue sections as usual.
    At the usual blocking step, block with serum (from same species as the secondary antibody) for 30 min at room temperature.
    Wash 3 X 2 min with PBS Tween 20.
    Incubate sections with an unconjugated AffiniPure Fab fragment Anti-Mouse IgG (H+L) for 1 hr at room temperature, or overnight 4°C . Try this blocking antibody at 0.1 mg/ml although the optimal concentration will need to be assessed by the end user.Proceed with antibody staining.




    2. Blocking endogenous Fc receptors
    There are kits available for this which use F(ab) monomeric secondary antibodies.

    Other tips that can be used to decrease general background:


    Incubate sections with 1% Triton (in PBS) at room temperature for 30 min to ‘clean’ the tissue.
    Use TBS –Tween 20 as a washing buffer. This often gives less background than using PBS-Tween 20.

    Read More

    Abcam Scientific Support

    回复于 Jan 31 2013

    Question

    1. The concentration of sheep serum used is 1:1000, diluted in PBS+1% TritonX. We block at room temperature for 90 minutes.

    2. Antigen retrieval was performed at 96C for 25 minutes. We haven’t performed a time series, we just went off several methods which recommended 25 minutes for antigen retrieval. There was no difference between the two pHs. The pH 10 buffer we used was in fact a Tris based buffer, not a citrate based buffer.

    3. We haven’t tested the secondary antibody with known primary antibodies which work as yet, as our lab has limited primary antibodies raised in mouse. We do however have a goat-anti-mouse fluorescent antibody which does work, and we will be trying this in the near future.



    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 02 2012

    Answer

    Thank you for your reply.


    I am sorry to hear that you have difficulties getting satisfactory results form this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this case, I would like to offer some suggestions to help optimize the results from ab668.

    1.) I suggest to decrease the amount of Triton X used in the buffers. 0.025% Triton X-100 is used in standard protocols. Triton X is a quite harsh detergent.

    2.) Secondary anti pan IgG antibodies sometimes bind some isotypes better than others. I therefore suggest to try a different secondary antibody and use an IgG1 specific antibody.

    Please let me know how you are getting on.

    Read More

    Abcam Scientific Support

    回复于 Oct 02 2012

    1-10 of 31 Abreviews or Q&A

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