重组Anti-Cytochrome C Oxidase subunit VIc/COX6C抗体[EPR9938] (ab150422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9938] to Cytochrome C Oxidase subunit VIc/COX6C
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytochrome C Oxidase subunit VIc/COX6C抗体[EPR9938]
参阅全部 Cytochrome C Oxidase subunit VIc/COX6C 一抗 -
描述
兔单克隆抗体[EPR9938] to Cytochrome C Oxidase subunit VIc/COX6C -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Cytochrome C Oxidase subunit VIc/COX6C aa 50-150 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: Human fetal heart lysate; HepG2 and A-673 cell lysates. Human heart lysates, Mouse heart lysates and Rat heart tissue lysates IHC-P: Human colon, breast carcinoma, colonic adenocarcinoma, kidney, liver, skeletal muscle and stomach tissues. Mouse cardiac muscle tissue, and Rat liver tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9938 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150422于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 9 kDa. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. |
靶标
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功能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
序列相似性
Belongs to the cytochrome c oxidase subunit 6c family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1345 Human
- Entrez Gene: 12864 Mouse
- Entrez Gene: 54322 Rat
- Omim: 124090 Human
- SwissProt: P09669 Human
- SwissProt: Q9CPQ1 Mouse
- SwissProt: P11951 Rat
- Unigene: 351875 Human
see all -
别名
- COX 6C antibody
- Cox6c antibody
- COX6C_HUMAN antibody
see all
图片
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All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/1000 dilution (Purified)
Lane 1 : Human heart lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted? -
Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/5000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1:500 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cardiac muscle tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1:500 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1:500 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/1000 dilution
Lane 1 : Human fetal heart cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : A-673 (Human muscle Ewing's Sarcoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 9 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Cytochrome C Oxidase subunit VIc/COX6C with ab150422 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Cytochrome C Oxidase subunit VIc/COX6C with ab150422 at 1/50 dilution.
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Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using ab150422 showing positive staining.
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Immunohistochemical analysis of paraffin embedded human liver tissue using ab150422 showing positive staining.
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Immunohistochemical analysis of paraffin embedded human colonic adenocarcinoma tissue using ab150422 showing positive staining.
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Immunohistochemical analysis of paraffin embedded human colon tissue using ab150422 showing positive staining.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab150422 被引用在 6 文献中.
- Li S et al. The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein. Sci Rep 11:14001 (2021). PubMed: 34234233
- Liu Y et al. Effect of TFAM on ATP content in tachypacing primary cultured cardiomyocytes and atrial fibrillation patients. Mol Med Rep 22:5105-5112 (2020). PubMed: 33174030
- Samuel P et al. Cisplatin induces the release of extracellular vesicles from ovarian cancer cells that can induce invasiveness and drug resistance in bystander cells. Philos Trans R Soc Lond B Biol Sci 373:N/A (2018). PubMed: 29158318
- Maiti AK et al. Colonic levels of vasoactive intestinal peptide decrease during infection and exogenous VIP protects epithelial mitochondria against the negative effects of IFN? and TNFa induced during Citrobacter rodentium infection. PLoS One 13:e0204567 (2018). PubMed: 30252907
- Bewicke-Copley F et al. Extracellular vesicles released following heat stress induce bystander effect in unstressed populations. J Extracell Vesicles 6:1340746 (2017). PubMed: 28717426
- Maiti AK et al. IL-4 Protects the Mitochondria Against TNFa and IFN? Induced Insult During Clearance of Infection with Citrobacter rodentium and Escherichia coli. Sci Rep 5:15434 (2015). IHC ; Mouse . PubMed: 26481427